Klebanoff S J, Durack D T, Rosen H, Clark R A
Infect Immun. 1977 Jul;17(1):167-73. doi: 10.1128/iai.17.1.167-173.1977.
A number of functional studies were performed on essentially pure eosinophil preparations obtained from the ascitic fluid of a patient with eosinophilic gastroenteritis. These cells responded to chemotactic factors including a bacterial factor, partially purified C5a, and factors generated from serum or ascitic fluid. The chemotactic activity generated in the patient's ascitic fluid was capable of attracting both neutrophils and eosinophils, was dependent on the presence of complement components, and was identified as C5a. Metabolic studies demonstrated that particle ingestion by eosinophils was associated with a marked increase in hexose monophosphate shunt activity ([1-14C]glucose oxidation), H2O2 formation ([14C]formate oxidation), superoxide anion generation, chemiluminescence, thyroid hormone degradation, iodination, and estrogen binding. This postphagocytic metabolic burst by eosinophils was qualitatively similar to that observed in neutrophils, but for several parameters the eosinophil response was greater than the neutrophil response.
对从一名嗜酸性粒细胞性胃肠炎患者腹水中获得的基本纯净的嗜酸性粒细胞制剂进行了多项功能研究。这些细胞对趋化因子有反应，包括一种细菌因子、部分纯化的C5a以及从血清或腹水中产生的因子。患者腹水中产生的趋化活性能够吸引中性粒细胞和嗜酸性粒细胞，依赖于补体成分的存在，并被鉴定为C5a。代谢研究表明，嗜酸性粒细胞摄取颗粒与己糖磷酸旁路活性（[1-14C]葡萄糖氧化）、H2O2形成（[14C]甲酸氧化）、超氧阴离子生成、化学发光、甲状腺激素降解、碘化和雌激素结合的显著增加有关。嗜酸性粒细胞的这种吞噬后代谢爆发在性质上与中性粒细胞中观察到的相似，但在几个参数上，嗜酸性粒细胞的反应大于中性粒细胞的反应。
