Department of Nutritional Sciences, University of Vienna, Vienna, Austria.
Free Radic Res. 2009 Aug;43(8):753-60. doi: 10.1080/10715760903040628.
The major aims of this study were to investigate the effect of an Ironman triathlon on DNA migration in the single cell gel electrophoresis assay, apoptosis and necrosis in the cytokinesis-block micronucleus cytome assay with lymphocytes and on changes of total antioxidant capacity in plasma. Blood samples were taken 2 days (d) before, within 20 min, 1 d, 5 d and 19 d post-race. The level of strand breaks decreased (p<0.05) immediately after the race, then increased (p<0.01) 1 d post-race and declined (p<0.01) until 19 d post-race. Apoptotic and necrotic cells decreased (p<0.01) and the total antioxidant status increased (p<0.01) immediately after the race. The results indicate that ultra-endurance exercise does not cause prolonged DNA damage in well-trained male athletes.
本研究的主要目的是研究铁三运动对单细胞凝胶电泳检测中 DNA 迁移、细胞有丝分裂阻断微核细胞胞质试验中细胞凋亡和坏死以及血浆总抗氧化能力变化的影响。在比赛前 2 天（d）、比赛后 20 分钟内、1 天、5 天和 19 天采集血样。赛后即刻，链断裂水平下降（p<0.05），随后 1 天增加（p<0.01），直至 19 天下降（p<0.01）。凋亡和坏死细胞减少（p<0.01），总抗氧化状态增加（p<0.01）。结果表明，超耐力运动不会导致训练有素的男性运动员的 DNA 损伤持续时间延长。
