Medrano A, Holt W V, Watson P F
Department of Veterinary Basic Sciences, Royal Veterinary College, London, UK.
Andrologia. 2009 Aug;41(4):246-50. doi: 10.1111/j.1439-0272.2009.00927.x.
Boar spermatozoa from different males were frozen at a number of cooling rates using a controlled-rate freezing machine designed to minimise thermal variables involved in the cooling process, to see whether inter-boar sperm cryosurvival may be improved by changing cooling rate. Four cooling rates in the range 3 degrees C min(-1) to 24 degrees C min(-1) from +5 degrees C to -5 degrees C and five cooling rates in the range 5 degrees C min(-1) to 80 degrees C min(-1) from -5 degrees C to -80 degrees C were tested. Motile spermatozoa were assessed by CASA, plasma membrane integrity by fluorescent probes (SYBR14/propidium iodide) and flow cytometry, and acrosome membrane integrity by lectins (PSA-rhodamine) and fluorescent microscopy. Cooling rate affected sperm cryosurvival from different boars in different ways; that is, spermatozoa from some individuals were less susceptible than those from others. For some individuals, sperm cryosurvival was poor regardless of cooling rate, but for others it was better with faster rates. This confirms cooling rate effects on sperm cryosurvival depend on inter-individual boar differences more than on the cooling process itself.
使用一台控速冷冻机，以多种降温速率对来自不同公猪的精子进行冷冻，该冷冻机旨在尽量减少降温过程中涉及的热变量，以探究改变降温速率是否能提高公猪间精子的冷冻存活率。测试了从+5℃至-5℃范围内3℃每分钟（-1）至24℃每分钟（-1）的四种降温速率，以及从-5℃至-80℃范围内5℃每分钟（-1）至80℃每分钟（-1）的五种降温速率。通过计算机辅助精子分析（CASA）评估活动精子，通过荧光探针（SYBR14/碘化丙啶）和流式细胞术评估质膜完整性，通过凝集素（PSA-罗丹明）和荧光显微镜评估顶体膜完整性。降温速率以不同方式影响不同公猪精子的冷冻存活率；也就是说，某些个体的精子比其他个体的精子更不易受影响。对于某些个体，无论降温速率如何，精子冷冻存活率都很低，但对于其他个体，降温速率越快存活率越高。这证实了降温速率对精子冷冻存活率的影响更多地取决于公猪个体间的差异，而非降温过程本身。
