INRA, UR50, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne, France.
J Appl Microbiol. 2010 Jan;108(1):325-40. doi: 10.1111/j.1365-2672.2009.04427.x.
The microbial diversity of bioaerosols released during operational activities at composting plants is poorly understood. Identification of bacteria and fungi present in such aerosols is the prerequisite for the definition of microbial indicators that could be used in dispersal and exposure studies.
A culture-independent analysis of composting bioaerosols collected at five different industrial open sites during the turning of composting piles in fermentation was performed by building 16S rDNA and 18S rDNA libraries. More than 800 sequences were analysed. Although differences in the phylotypes distribution were observed from one composting site to another, similarities in the structure of microbial diversity were remarkable. The same phyla dominated in the five bioaerosols: Ascomycota among fungi, Firmicutes and Actinobacteria among bacteria. For each phylum, some dominant phylotypes were common to at least four bioaerosols. These common phylotypes belonged to Thermomyces, Aspergillus, Penicillium, Geobacillus, Planifilum, Thermoactinomyces, Saccharopolyspora, Thermobifida and Saccharomonospora.
The microbial signature of aerosols produced during the thermophilic phase of composting was determined. The similarities observed may be explained by the selection of thermophilic and sporulating species.
Several bacteria and fungi identified in this study may represent potential indicators of composting bioaerosols in air.
在堆肥厂的运行活动中释放的生物气溶胶的微生物多样性了解甚少。识别此类气溶胶中存在的细菌和真菌是定义可用于扩散和暴露研究的微生物指标的前提。
通过构建 16S rDNA 和 18S rDNA 文库，对发酵过程中在五个不同工业开放场地的堆肥堆翻堆时收集的堆肥生物气溶胶进行了非培养分析。分析了 800 多个序列。尽管从一个堆肥场到另一个堆肥场观察到了分类群分布的差异，但微生物多样性结构的相似性非常显著。相同的门在五个生物气溶胶中占主导地位：真菌中的子囊菌门，细菌中的厚壁菌门和放线菌门。对于每个门，至少有四个生物气溶胶中存在一些共同的优势分类群。这些共同的分类群属于嗜热丝孢酵母属、曲霉属、青霉属、芽孢杆菌属、Planifilum、嗜热放线菌属、糖多孢菌属、Thermobifida 和 Saccharomonospora。
确定了堆肥高温阶段产生的气溶胶的微生物特征。观察到的相似性可能是由于对嗜热和孢子形成物种的选择。
本研究中鉴定的几种细菌和真菌可能代表空气中堆肥生物气溶胶的潜在指标。
