Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, Korea.
Phytother Res. 2010 Feb;24(2):198-204. doi: 10.1002/ptr.2945.
Danshen is widely used in traditional Chinese medicine, often in combination with other herbs. To check the effect of Danshen on the proliferation of breast cancer cells, Danshen extract was used to treat MCF-7 and MCF-7 HER2 cells, the latter of which overexpresses HER2. HER2 is a receptor tyrosine kinase, and is involved in signal transduction pathways leading to tumor cell proliferation. MTT and cell proliferation assays revealed that Danshen strongly inhibited the proliferation of both MCF-7 vec cells and MCF-7 HER2 cells. Flow cytometry analyses indicated that Danshen induced cell cycle delay in the G1 phase. HER2 expression was shown to confer resistance to Danshen-induced inhibition of proliferation and cell cycle delay, suggesting that HER2 is responsible for the resistance to Danshen. Danshen treatment induced the down-regulation of Akt phosphorylation and an increase in p27 in MCF-7 vec and MCF-7 HER2 cells. Nevertheless, MCF-7 HER2 cells were more resistant to the Danshen-induced inhibition of Akt phosphorylation and p27 up-regulation.
丹参在中药中被广泛应用，常与其他草药联合使用。为了考察丹参对乳腺癌细胞增殖的影响，我们用丹参提取物处理 MCF-7 和 MCF-7 HER2 细胞，后者过表达 HER2。HER2 是一种受体酪氨酸激酶，参与导致肿瘤细胞增殖的信号转导途径。MTT 和细胞增殖实验表明，丹参强烈抑制 MCF-7 vec 细胞和 MCF-7 HER2 细胞的增殖。流式细胞术分析表明，丹参诱导细胞周期在 G1 期停滞。HER2 的表达赋予了对丹参诱导的增殖抑制和细胞周期阻滞的抗性，表明 HER2 是产生抗性的原因。丹参处理诱导 MCF-7 vec 和 MCF-7 HER2 细胞中 Akt 磷酸化下调和 p27 增加。然而，MCF-7 HER2 细胞对丹参诱导的 Akt 磷酸化抑制和 p27 上调更具抗性。
