Faller D V, Hopkins N
J Virol. 1977 Jul;23(1):188-95. doi: 10.1128/JVI.23.1.188-195.1977.
We used two-dimensional gel electrophoresis to obtain fingerprints of RNase T1-resistant oligonucleotides of a B-tropic murine leukemia virus from BALB/c and five NB-tropic viruses independently derived from this B virus by passage through NIH Swiss mouse embryo cells in vitro. The fingerprints of the B- and NB-tropic viruses were very similar: approximately 33 of 35 large T1-resistant oligonucleotides appeared to be shared by these viruses. However, the five NB-tropic viruses possessed an apparently common alteration relative to their B virus progenitor. This change involved the acquisition of one oligonucleotide and, tentatively, the loss of one oligonucleotide. We do not know whether these changes represent an alteration responsible for the change from B- to NB-tropism. Fingerprints of B- and NB-tropic viruses were not affected when the viruses were grown in cells of different Fv-1 type.
我们使用二维凝胶电泳来获取来自BALB/c的B嗜性鼠白血病病毒以及通过体外传代于NIH瑞士小鼠胚胎细胞而独立衍生自该B病毒的五种NB嗜性病毒的RNase T1抗性寡核苷酸指纹图谱。B嗜性和NB嗜性病毒的指纹图谱非常相似：这些病毒似乎共有约35个大的T1抗性寡核苷酸中的33个。然而，相对于它们的B病毒祖代，这五种NB嗜性病毒具有一个明显共同的改变。这种变化涉及获得一个寡核苷酸，并初步认为是失去一个寡核苷酸。我们不知道这些变化是否代表了导致从B嗜性转变为NB嗜性的改变。当病毒在不同Fv-1类型的细胞中生长时，B嗜性和NB嗜性病毒的指纹图谱不受影响。
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