Faller D V, Hopkins N
J Virol. 1977 Nov;24(2):609-17. doi: 10.1128/JVI.24.2.609-617.1977.
We used two-dimensional gel electrophoresis to obtain fingerprints of 32P-labeled RNase T1-resistant oligonucleotides derived from the genomes of an N- and a B-tropic murine leukemia virus of BALB/c. These viruses share approximately 30 large T1-resistant oligonucleotides. In addition, there are eight large oligonucleotides unique to the N-tropic virus, and there are six B-trophic virus-specific oligonucleotides. Viruses, designated XLP-N, which appear by biological criteria and analysis of virion proteins to be recombinants between these N- and B-tropic viruses, possess some but not all of the N or B virus-specific oligonucleotides.
我们使用二维凝胶电泳来获取源自BALB/c的N型和B型嗜性鼠白血病病毒基因组的32P标记的核糖核酸酶T1抗性寡核苷酸的指纹图谱。这些病毒共有约30个大的T1抗性寡核苷酸。此外,有8个大的寡核苷酸是N型嗜性病毒特有的,还有6个是B型嗜性病毒特有的寡核苷酸。通过生物学标准和病毒体蛋白质分析显示为这些N型和B型嗜性病毒之间重组体的病毒,命名为XLP-N,具有部分而非全部的N型或B型病毒特异性寡核苷酸。