Lau A F, Krzyzek R A, Brugge J S, Erikson R L, Schollmeyer J, Faras A J
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3904-8. doi: 10.1073/pnas.76.8.3904.
The biological and biochemical properties of Rous sarcoma virus-transformed and revertant field vole cells were investigated. Revertant vole cells appear morphologically similar to normal, uninfected cells, yet, like transformed vole cells, they are fully capable of growing in agar suspension and producing tumors in athymic nude mice. These highly tumorigenic, yet morphologically normal appearing, vole cells express viral-specific antigens such as the gag gene product (Pr76) but lack the env gene protein (gp85). Moreover, they contain the src gene protein, pp60src. These results support the concept of the pleiotropic nature of the src gene product and in addition suggest that pp60src may have multiply mechanisms of action. With this revertant cell system it may be feasible to distinguish between those biochemical functions of the src gene product that are important for tumorigenicity in vivo and those that are related to in vitro morphological transformation.
对劳氏肉瘤病毒转化的和回复突变的田鼠细胞的生物学和生物化学特性进行了研究。回复突变的田鼠细胞在形态上类似于正常的未感染细胞，然而，与转化的田鼠细胞一样，它们完全能够在琼脂悬浮液中生长并在无胸腺裸鼠中产生肿瘤。这些具有高度致瘤性但形态上看似正常的田鼠细胞表达病毒特异性抗原，如gag基因产物（Pr76），但缺乏env基因蛋白（gp85）。此外，它们含有src基因蛋白pp60src。这些结果支持src基因产物具有多效性的概念，此外还表明pp60src可能具有多种作用机制。利用这个回复突变细胞系统，区分src基因产物在体内对致瘤性重要的那些生化功能和与体外形态转化相关的那些生化功能可能是可行的。
