Department of Surgery, Klinikum Ludwigshafen, Germany.
Colorectal Dis. 2010 Dec;12(12):1249-53. doi: 10.1111/j.1463-1318.2009.02010.x.
The cause of haemorrhoidal disease is unknown, epidemiological data and histopathological findings support the hypothesis that reduced connective tissue stability is associated with the incidence of haemorrhoids. Therefore the aim of this study was to analyse the quantity and quality of collagen formation in the corpus cavernosum recti in patients with III°/IV° haemorrhoids in comparison with persons without haemorrhoids.
Haemorrhoidectomy specimens of 31 patients with III°/IV° haemorrhoids were examined. The specimens of 20 persons who died a natural death and who had no haemorrhoidal disease served as the controls. The amount of collagen was estimated photometrically by calculating the collagen/protein ratio. The collagen I/III ratio served as parameter for the quality of collagen formation and was calculated using cross polarization spectroscopy.
Patients with haemorrhoids had a significantly reduced collagen/protein ratio (42.2 ± 16.2μg/mg vs 72.5±31.0μg/mg; P= 0.02) and a significantly reduced collagen I/III ratio (2.0±0.1 vs 4.6±0.3; P<0.001) compared with persons without haemorrhoidal disease. There was no correlation with patients' age or gender.
There is a fundamental disorder of collagen metabolism in patients with haemorrhoidal disease. It remains unclear whether this is due to exogenous or endogenous influences.
痔疮的病因尚不清楚，流行病学数据和组织病理学发现支持这样一种假说，即结缔组织稳定性降低与痔疮的发生有关。因此，本研究旨在分析 III°/IV°痔疮患者直肠海绵体组织中胶原蛋白的形成数量和质量，并与无痔疮者进行比较。
对 31 例 III°/IV°痔疮患者的痔切除术标本进行了检查。20 例因自然原因死亡且无痔疮的患者的标本作为对照。通过计算胶原蛋白/蛋白比，用分光光度法估计胶原蛋白的量。胶原蛋白 I/III 比作为胶原蛋白形成质量的参数，并用偏振光光谱法计算。
与无痔疮的患者相比，痔疮患者的胶原蛋白/蛋白比（42.2±16.2μg/mg 比 72.5±31.0μg/mg；P=0.02）和胶原蛋白 I/III 比（2.0±0.1 比 4.6±0.3；P<0.001）显著降低。患者的年龄或性别与这些结果均无相关性。
痔疮患者的胶原蛋白代谢存在根本紊乱。目前尚不清楚这是外源性还是内源性影响所致。
