Laboratoire de Biologie de la Reproduction, Service d'Histologie-Embryologie-Cytogénétique, CHU Jean Verdier, Assistance Publique-Hôpitaux de Paris, Bondy, France.
Eur J Obstet Gynecol Reprod Biol. 2009 Nov;147(1):52-6. doi: 10.1016/j.ejogrb.2009.06.023. Epub 2009 Jul 16.
OBJECTIVE(S): We aimed to evaluate and compare the embryo quality at early cleavage stages using different oils overlaying media to culture human embryos during IVF/ICSI treatments.
A total of 500 IVF/ICSI treatments from 500 women were analyzed in a prospective randomized study. Oocytes/embryos were treated into microdroplets of appropriate media overlaid with (i) Mineral Oil (CryoBioSystem, L'Aigle, France) (group 1, n=129), (ii) Liquid Paraffin (Medicult, Lyon, France) (group 2, n=126), (iii) Nidoil (Nidacon International, Guthenburg, Sweden) (group 3, n=126) and (iv) Ovoil (Vitrolife, Kungsbacka, Sweden) (group 4, n=119). Comparisons between groups were done using two by two post hoc tests, with 5% significance. The primary endpoint was the embryo quality, defined as good or top quality when embryos were with (i) less than 20% of fragmentation and (ii) 3-5/4 cells at day 2 or 6-10/8 cells at day 3, respectively.
At day 2, the embryo quality was similar in all groups. However, the mean number of top quality embryos at day 3 was statistically higher into the group 4 (1.4+/-1.8) compared to the group 1 (0.9+/-1.0; p=0.03) and 2 (0.8+/-1.3; p=0.05). Furthermore, a significant increase of the mean number of good quality embryos was observed at day 3 into the group 4 (2.6+/-2.6) compared to the group 1 (1.6+/-1.6; p=0.02).
CONCLUSION(S): The embryo quality could be modified according to commercial oils used to overlay culture media.
我们旨在评估和比较在体外受精/卵胞浆内单精子注射（IVF/ICSI）治疗中使用不同油覆盖培养基培养人类胚胎时早期卵裂阶段的胚胎质量。
对 500 名女性的 500 个 IVF/ICSI 治疗进行了前瞻性随机研究。将卵母细胞/胚胎处理成适当培养基的微滴中，并用（i）矿物油（CryoBioSystem，L'Aigle，法国）（第 1 组，n=129），（ii）液体石蜡（Medicult，Lyon，法国）（第 2 组，n=126），（iii）Nidoil（Nidacon International，Guthenburg，瑞典）（第 3 组，n=126）和（iv）Ovoil（Vitrolife，Kungsbacka，瑞典）（第 4 组，n=119）覆盖。使用两两事后检验进行组间比较，显著性水平为 5%。主要终点是胚胎质量，当胚胎（i）碎片少于 20%和（ii）第 2 天 3-5/4 个细胞或第 3 天 6-10/8 个细胞时定义为优质或顶级质量。
第 2 天，所有组的胚胎质量相似。然而，第 3 天顶级优质胚胎的平均数量在第 4 组（1.4+/-1.8）中统计学上高于第 1 组（0.9+/-1.0；p=0.03）和第 2 组（0.8+/-1.3；p=0.05）。此外，第 3 天第 4 组优质胚胎的平均数量明显增加（2.6+/-2.6）与第 1 组（1.6+/-1.6；p=0.02）相比。
根据覆盖培养基的商业油，可以改变胚胎质量。
