Laboratory of Cancer Research MAKNA-UPM, Institute of Biosciences (IBS), Universiti Putra Malaysia, Serdang, Selangor DE 43400, Malaysia.
Evid Based Complement Alternat Med. 2011;2011:965370. doi: 10.1093/ecam/nep091. Epub 2011 Jun 5.
The use of evidence-based complementary and alternative medicine is increasing rapidly. Eleucine indica (EI) is traditionally used in ailments associated with liver and kidneys. The therapeutic benefit of the medicinal plants is often attributed to their antioxidant properties. Therefore, the aim of this study was to screen the hexane, dicholoromethane, ethyl acetate (EA) and methanol extracts (MeTH) of EI for their antioxidant, antibacterial and anti-cancer effects using total phenolic contents (TPCs) and DPPH, disc diffusion method and MTT cytotoxicity assays, respectively. The MeTH was showed to have the highest TPC and scavenging activity (77.7%) on DPPH assay, followed by EA (64.5%), hexane (47.19%) and DCM (40.83%) extracts, whereas the MeTH showed no inhibitory effect on all tested bacteria strains. However, the EA extract exhibited a broad spectrum antibacterial activity against all tested bacteria except Bacillus subtilis, in which this bacterium was found to be resistant to all EI extracts. Meanwhile, hexane extract was demonstrated to have a remarkable antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, while the dicholoromethane extract did not exhibit significant activity against P. aeruginosa. None of the extracts showed significant cytotoxic activity towards MCF-7, HT-29 and CEM-SS human cancer cell lines after 72 h incubation time (IC(50) > 30 μg/ml). These results demonstrate that the extract prepared from the EI possesses antioxidant activity in vitro in addition to antibacterial properties. Further investigations are needed to verify the antioxidant effects in vitro and in vivo.
基于证据的补充和替代医学的使用正在迅速增加。Eleucine indica（EI）传统上用于与肝脏和肾脏相关的疾病。药用植物的治疗益处通常归因于其抗氧化特性。因此，本研究的目的是使用总酚含量（TPC）和 DPPH、圆盘扩散法和 MTT 细胞毒性测定法分别筛选 EI 的己烷、二氯甲烷、乙酸乙酯（EA）和甲醇提取物（MeTH）的抗氧化、抗菌和抗癌作用。MeTH 显示出最高的 TPC 和对 DPPH 测定的清除活性（77.7%），其次是 EA（64.5%）、己烷（47.19%）和 DCM（40.83%）提取物，而 MeTH 对所有测试的细菌菌株均无抑制作用。然而，EA 提取物对所有测试的细菌表现出广谱抗菌活性，除了枯草芽孢杆菌，这种细菌对所有 EI 提取物都有抗性。同时，己烷提取物对耐甲氧西林金黄色葡萄球菌（MRSA）和铜绿假单胞菌表现出显著的抗菌活性，而二氯甲烷提取物对铜绿假单胞菌没有显著的活性。在 72 小时孵育时间后，没有一种提取物对 MCF-7、HT-29 和 CEM-SS 人癌细胞系表现出显著的细胞毒性活性（IC50>30μg/ml）。这些结果表明，从 EI 制备的提取物除了具有抗菌特性外，还具有体外抗氧化活性。需要进一步的研究来验证体外和体内的抗氧化作用。
