Advanced Photonics Center, Southeast University, Nanjing 210096, China.
Anal Bioanal Chem. 2012 Jan;402(3):1093-100. doi: 10.1007/s00216-011-5577-z. Epub 2011 Nov 29.
The detection and identification of epidermal growth factor receptor 2 (HER2)-positive breast cancer cells is crucial for the clinic therapy of breast cancer. For the aim of the detection, a novel surface-enhanced Raman scattering (SERS) probe for distinguishing breast cancers at different HER2 statuses is reported in this paper. In such a probe, anti-HER2 antibody-conjugated silver nanoparticles have been synthesized for specific targeting of HER2-positive breast cancer cells. More importantly, different from the previously reported SERS probe for targeting cancer cells, p-mercaptobenzoic acid is utilized as both the Raman reporter and the conjugation agent for attaching antibody molecules, which leads to a much simplified structure. For investigating the ability of such a probe to distinguish breast cancer cells, SKBR3 and MCF7 cells were chosen as two model systems, which are HER2-positive- and HER2-negative-expressing cells, respectively. The experimental results reveal that SKBR3 cells exhibit much stronger SERS signals than MCF7 cells, indicating that the probe could be utilized to distinguish breast cancer cells at different HER2 statuses. This kind of SERS probe holds a potential for a direct detection of living breast cancer cells with the advantages of easy fabrication, high SERS sensitivity, and biocompatibility.
表皮生长因子受体 2（HER2）阳性乳腺癌细胞的检测和鉴定对于乳腺癌的临床治疗至关重要。为了实现这一检测目的，本文报道了一种用于区分不同 HER2 状态乳腺癌的新型表面增强拉曼散射（SERS）探针。在该探针中，合成了抗 HER2 抗体偶联的银纳米颗粒，用于特异性靶向 HER2 阳性乳腺癌细胞。更重要的是，与之前报道的用于靶向癌细胞的 SERS 探针不同，本文中使用了对巯基苯甲酸作为拉曼报告分子和连接剂来连接抗体分子，从而大大简化了结构。为了研究该探针区分乳腺癌细胞的能力，选择了 SKBR3 和 MCF7 细胞作为两种模型系统，它们分别是 HER2 阳性和 HER2 阴性表达细胞。实验结果表明，SKBR3 细胞表现出比 MCF7 细胞更强的 SERS 信号，表明该探针可用于区分不同 HER2 状态的乳腺癌细胞。这种 SERS 探针具有直接检测活乳腺癌细胞的潜力，具有易于制备、高 SERS 灵敏度和生物相容性等优点。
