LM-UGent, Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
BCCM/LMG Bacteria Collection, Ghent University, Ghent, Belgium.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1457-1464. doi: 10.1099/ijs.0.032615-0. Epub 2011 Aug 12.
Several Gram-negative-staining, facultatively anaerobic bacterial isolates were obtained from Eucalyptus seedlings showing symptoms of bacterial blight and dieback in Colombia, Rwanda and South Africa. Partial 16S rRNA gene sequencing, together with partial gyrB sequencing, placed the isolates in the genus Pantoea and indicated that they constituted three novel species. Multilocus sequence analysis (MLSA) based on partial sequences of gyrB, rpoB, infB and atpD revealed Pantoea dispersa, Pantoea eucrina and Pantoea cypripedii as their closest phylogenetic relatives. DNA-DNA hybridization studies confirmed the classification of the new isolates as three novel species and phenotypic tests allowed them to be differentiated from their closest phylogenetic neighbours. The names Pantoea rodasii sp. nov. [type strain LMG 26273(T)=BD 943(T) (deposited with the Plant Pathogenic and Plant Protecting Bacteria Collection, South Africa)=BCC 581(T) (deposited with the Bacterial Culture Collection, Forestry and Agricultural Institute, South Africa)], Pantoea rwandensis sp. nov. (type strain LMG 26275(T)=BD 944(T)=BCC 571(T)) and Pantoea wallisii sp. nov. (type strain LMG 26277(T)=BD 946(T)=BCC 682(T)) are proposed.
从表现出细菌性枯萎和衰退症状的桉树幼苗中分离到了一些革兰氏阴性需氧细菌分离株，这些分离株来自哥伦比亚、卢旺达和南非。部分 16S rRNA 基因测序和部分 gyrB 测序将这些分离株归入潘多拉属，并表明它们构成了三个新物种。基于部分 gyrB、rpoB、infB 和 atpD 序列的多位点序列分析（MLSA）显示，潘多拉分散菌、潘多拉真养菌和潘多拉鸢尾菌是它们最接近的系统发育近亲。DNA-DNA 杂交研究证实了新分离株的分类为三个新物种，表型测试允许它们与最接近的系统发育近缘种区分开来。将 Pantoea rodasii sp. nov. [模式株 LMG 26273(T)=BD 943(T)（保藏于南非植物病原和植物保护细菌收藏中心）=BCC 581(T)（保藏于南非林业和农业研究所细菌培养收藏中心）]、Pantoea rwandensis sp. nov.（模式株 LMG 26275(T)=BD 944(T)=BCC 571(T)）和 Pantoea wallisii sp. nov.（模式株 LMG 26277(T)=BD 946(T)=BCC 682(T)）命名为新种。
