Pediatric Critical Care Unit, the Division of Hematology-Oncology, the Department of Biochemistry, Sainte-Justine Hospital and Université de Montréal, Montréal, Canada.
Transfusion. 2009 Nov;49(11):2326-34. doi: 10.1111/j.1537-2995.2009.02319.x. Epub 2009 Jul 17.
The relationship between length of storage of red blood cell (RBC) units and biochemical changes has been well studied, but little is known about the progression of cellular immunomodulative properties in blood recipients. This study aims to quantify in vitro T-cell activation and cytokine release by white blood cells, after incubation with supernatants from leukoreduced RBCs.
Whole blood cultures were incubated with supernatant from five leukoreduced RBC units stored for 1, 6, 10, 15, 24, and 42 days. Supernatant-induced T-cell activation was evaluated by quantifying CD25 expression. Supernatant-induced cytokine production was determined by measuring interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-alpha levels.
No cytokines were detected in RBC supernatants even after 42 days of storage. However, IL-6 levels in whole blood culture increased significantly when incubated with supernatant from RBC units stored for 1, 6, and 15 days, by factors of 1.7 +/- 0.3, 1.7 +/- 0.3, and 1.4 +/- 0.3, respectively. TNF-alpha levels were significantly decreased on Days 24 and 42 of storage by factors of 0.50 +/- 0.42 and 0.33 +/- 0.21, respectively. IL-10 levels were significantly increased on Days 1 and 42 of storage by factors of 2.3 +/- 1.3 and 3.2 +/- 2.8, respectively. After an initial increase in IL-6 and TNF-alpha production, there was a significant linear decrease in their levels measured from units stored for longer times. No significant changes in CD25 expression were observed over time.
Although no cytokines were measured in the supernatants from leukoreduced RBCs, these supernatants exhibited variable immunomodulatory effects related to their length of storage.
已对红细胞（RBC）单位储存时间与生化变化之间的关系进行了深入研究，但对于血液接受者中细胞免疫调节特性的进展知之甚少。本研究旨在定量检测白细胞与经过白细胞减少处理的 RBC 上清液孵育后 T 细胞的体外激活和细胞因子释放。
将全血培养物与储存 1、6、10、15、24 和 42 天的 5 个白细胞减少 RBC 单位的上清液孵育。通过定量检测 CD25 表达来评估上清液诱导的 T 细胞激活。通过测量白细胞介素（IL）-6、IL-10 和肿瘤坏死因子（TNF）-α水平来确定上清液诱导的细胞因子产生。
即使在 RBC 上清液储存 42 天后，也未检测到细胞因子。然而，当用储存 1、6 和 15 天的 RBC 单位的上清液孵育全血培养物时，IL-6 水平分别显著增加了 1.7 ± 0.3、1.7 ± 0.3 和 1.4 ± 0.3 倍。TNF-α水平在储存第 24 天和第 42 天分别显著下降了 0.50 ± 0.42 和 0.33 ± 0.21 倍。IL-10 水平在储存第 1 天和第 42 天分别显著增加了 2.3 ± 1.3 和 3.2 ± 2.8 倍。在最初的 IL-6 和 TNF-α产生增加之后，测量来自储存时间较长的单位的这些细胞因子水平呈显著线性下降。CD25 表达随时间无明显变化。
尽管在白细胞减少的 RBC 上清液中未测量到细胞因子，但这些上清液表现出与储存时间相关的可变免疫调节作用。
