Paier Anton, Agewall Stefan, Kublickiene Karolina
Institution for Clinical Science Intervention and Technology , Division of Obstetrics and Gynecology, Karolinska Institutet, Karolinska University Hospital, Huddinge Campus, Stockholm 14186, Sweden.
Heart Vessels. 2009 Jul;24(4):260-6. doi: 10.1007/s00380-008-1117-y. Epub 2009 Jul 22.
Heat shock proteins (HSPs) have been suggested to play an important role in the pathogenesis of cardiovascular disease; however, their levels in resistance arteries and their role as useful markers for endothelial dysfunction are not well known. In this paper we studied the levels of HSP90, HSP70, HSP60, HSP27, and of the oxidative stress marker nitrotyrosine (NT) in isolated small subcutaneous arteries from female and male patients with coronary heart disease (CHD) and compared them with healthy controls. HSPs and NT levels were analyzed by immunohistochemistry (IHC) with streptavidin-biotin complex and 3,3'-diaminobenzidine (DAB) staining. The results were assessed with a semi-quantitative method. The study showed lower levels of HSP90 in arteries from both male and female patients when compared to the healthy controls, while levels of HSP70 were lower only in male patients versus controls. The levels of HSP60 and HSP27 did not show any significant difference in either the male or the female groups. NT levels were higher in the arteries from female patients as compared to controls. In conclusion, the present study strengthens the concept that HSPs may play an important role in the pathogenesis of CHD, and that at least two of them, HSP70 and HSP90, may have useful applications as markers of vascular dysfunction in resistance arteries.
热休克蛋白（HSPs）被认为在心血管疾病的发病机制中起重要作用；然而，它们在阻力动脉中的水平以及作为内皮功能障碍有用标志物的作用尚不清楚。在本文中，我们研究了冠心病（CHD）女性和男性患者分离的皮下小动脉中HSP90、HSP70、HSP60、HSP27以及氧化应激标志物硝基酪氨酸（NT）的水平，并将其与健康对照进行比较。采用链霉亲和素-生物素复合物和3,3'-二氨基联苯胺（DAB）染色的免疫组织化学（IHC）方法分析HSPs和NT水平。结果采用半定量方法进行评估。研究表明，与健康对照相比，男性和女性患者动脉中的HSP90水平均较低，而与对照相比，只有男性患者的HSP70水平较低。HSP60和HSP27水平在男性或女性组中均未显示出任何显著差异。与对照相比，女性患者动脉中的NT水平较高。总之，本研究强化了HSPs可能在CHD发病机制中起重要作用的概念，并且其中至少两种，即HSP70和HSP90，可能作为阻力动脉血管功能障碍的标志物具有有用的应用价值。
