College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Int J Syst Evol Microbiol. 2009 Dec;59(Pt 12):3058-62. doi: 10.1099/ijs.0.008763-0. Epub 2009 Jul 30.
A novel golden-yellow-coloured strain, designated RHYL-37(T), was isolated from a soil sample from a forest of Euphrates poplar (Populus euphratica) in Xinjiang, China. The cells were strictly aerobic, Gram-staining-negative, heterotrophic, non-spore-forming rods that were motile by gliding. Growth occurred at 17-37 degrees C (optimum, 30 degrees C), at pH 5.0-9.0 (optimum, pH 7.0) and with 0-1 % NaCl (w/v; optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolate formed a cluster with members of the genera Segetibacter, Terrimonas, Niastella, Niabella and Chitinophaga in the family 'Chitinophagaceae' in the phylum Bacteroidetes. Sequence similarities with the type strains of recognized species ranged from 90.6 to 94.0 %. The predominant isoprenoid quinone was MK-7 and the major cellular fatty acids were iso-C(15 : 0), iso-C(15 : 1), C(16 : 0) and iso-C(17 : 0) 3-OH. The DNA G+C content was 39.7 mol%. On the basis of the phylogenetic, physiological and chemotaxonomic data, strain RHYL-37(T) represents a novel genus and species in the family 'Chitinophagaceae', for which the name Parasegetibacter luojiensis gen. nov., sp. nov. is proposed. The type strain of Parasegetibacter luojiensis is RHYL-37(T) (=CCTCC AB 208240(T)=KCTC 22561(T)).
一株新颖的金黄色菌株，命名为 RHYL-37(T)，从中国新疆胡杨（Populus euphratica）森林中的土壤样本中分离得到。细胞为严格需氧、革兰氏染色阴性、异养、非孢子形成的杆状，通过滑行运动。生长发生在 17-37°C（最佳温度 30°C）、pH 5.0-9.0（最佳 pH 7.0）和 0-1%NaCl（w/v；最佳 0%）。基于 16S rRNA 基因序列的系统发育分析表明，新分离株与属 Segetibacter、Terrimonas、Niastella、Niabella 和 Chitinophaga 中的成员形成一个聚类在拟杆菌门的 'Chitinophagaceae' 科中。与公认种的模式菌株的序列相似性范围为 90.6-94.0%。主要的异戊二烯醌是 MK-7，主要的细胞脂肪酸是 iso-C(15:0)、iso-C(15:1)、C(16:0)和 iso-C(17:0)3-OH。DNA G+C 含量为 39.7mol%。基于系统发育、生理和化学分类学数据，菌株 RHYL-37(T)代表 Chitinophagaceae 科中的一个新属和新种，因此提议 Parasegetibacter luojiensis 属，新种。Parasegetibacter luojiensis 的模式株为 RHYL-37(T)=CCTCC AB 208240(T)=KCTC 22561(T)。
