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[通过电泳分型检测轮状病毒感染链]

[Detection of rotavirus infection chains by electropherotyping].

作者信息

Meibohm K U, Nehrkorn R, Steinmann J

机构信息

Abteilung für Klinische Virologie, Staatliches Hygiene-Institut, Bremen.

出版信息

Monatsschr Kinderheilkd. 1990 Nov;138(11):749-54.

PMID:1963208
Abstract

518 fecal specimens were examined by SDS-PAGE after rotavirus had been detected by an ELISA. The material was collected from 239 patients in three children's hospitals in Bremen over a period of one year. Seventy-two percent of the children had a nosocomial infection. Eight electropherotypes could be distinguished by electrophoresis, one of which (type C) dominated (61.1%). In the neonatal wards of the children's hospitals one main electropherotype (type C) was detected throughout the year. In contrast, different electropherotypes could be detected simultaneously in the isolation wards. Some other electropherotypes were found only in certain hospitals. Our examinations demonstrate that by these additional electrophoretic investigations infection chains within children's hospitals can be detected and that the endemic occurrence of rotavirus in neonatal wards is less dependent on those types circulating in the general population.

摘要

在用ELISA检测出轮状病毒后,对518份粪便标本进行了SDS-PAGE检测。这些标本是在一年时间里从不来梅市三家儿童医院的239名患者中收集的。72%的儿童患有医院感染。通过电泳可区分出8种电泳型,其中一种(C型)占主导(61.1%)。在儿童医院的新生儿病房,全年都检测到一种主要的电泳型(C型)。相比之下,在隔离病房可同时检测到不同的电泳型。其他一些电泳型仅在某些医院被发现。我们的检测表明,通过这些额外的电泳研究,可以检测出儿童医院内的感染链,并且新生儿病房中轮状病毒的地方性流行较少依赖于在普通人群中传播的那些类型。

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