Carter Robert, Drouin Guy
Département de Biologie et Centre de Recherche Avancée en Génomique Environnementale, Université d'Ottawa, Ottawa, Ontario, Canada.
Mol Biol Evol. 2009 Nov;26(11):2515-20. doi: 10.1093/molbev/msp164. Epub 2009 Jul 24.
A defining characteristic of all eukaryotes is the presence of three RNA polymerases, each of which transcribes a particular subset of nuclear genes. RNA polymerase I transcribes rRNA genes; RNA polymerase II transcribes mRNA, miRNA, snRNA, and snoRNA genes; and RNA polymerase III transcribes 5S rRNA and tRNA genes. Here, we use the sequences of up to 25 Ascomycete species to show that the type of genes transcribed by each RNA polymerase affects their evolutionary rates and those of their transcription factors (TFs). The RNA polymerase subunits and TFs of genes whose promoters experience higher levels of concerted evolution evolve significantly faster than those experiencing lower levels of concerted evolution. The rates of evolution of RNA polymerase genes and their TFs are therefore not only the result of diverse selective constraints but are also influenced by the level of concerted evolution of the genes they transcribe.
所有真核生物的一个决定性特征是存在三种RNA聚合酶，每种聚合酶转录特定的核基因子集。RNA聚合酶I转录rRNA基因；RNA聚合酶II转录mRNA、miRNA、snRNA和snoRNA基因；RNA聚合酶III转录5S rRNA和tRNA基因。在这里，我们使用多达25种子囊菌物种的序列来表明，每种RNA聚合酶转录的基因类型会影响它们的进化速率以及它们转录因子（TFs）的进化速率。其启动子经历更高水平协同进化的基因的RNA聚合酶亚基和TFs，比经历较低水平协同进化的基因进化得明显更快。因此，RNA聚合酶基因及其TFs的进化速率不仅是多种选择限制的结果，还受到它们所转录基因的协同进化水平的影响。
