Department of Chemical and Biomolecular Engineering (BK21 Program), Institute for the BioCentury, KAIST, Daejeon 305-701, Korea.
Bioprocess Biosyst Eng. 2010 Jan;33(1):21-30. doi: 10.1007/s00449-009-0354-5. Epub 2009 Jul 28.
In this study, we present in vitro cytotoxicity of iron oxide (Fe(3)O(4)) and manganese oxide (MnO) using live/dead cell assay, lactate dehydrogenase assay, and reactive oxygen species detection with variation of the concentration of nanoparticles (5-500 microg/ml), incubation time (18-96 h), and different human cell lines (lung adenocarcinoma, breast cancer cells, and glioblastoma cells). The surface of nanoparticles is modified with polyethyleneglycol-derivatized phospholipid to enhance the biocompatibility, water-solubility, and stability under an aqueous media. While the cytotoxic effect was negligible for 18 h incubation even at highest concentration of 500 microg/ml, MnO nanoparticle represented higher level of toxicity than those of Fe(3)O(4) and the commercial medical contrast reagent, Feridex after 2 and 4 day incubation time. However, the cytotoxicity of Fe(3)O(4) is equivalent or better than Feridex based on the live/dead cell viability assay. The engineered MnO and Fe(3)O(4) exhibited excellent stability compared with Feridex for a prolonged incubation time.
在这项研究中，我们使用活/死细胞检测、乳酸脱氢酶检测和活性氧检测，研究了氧化铁 (Fe(3)O(4)) 和氧化锰 (MnO) 的体外细胞毒性，纳米颗粒浓度 (5-500μg/ml)、孵育时间 (18-96 h) 和不同人类细胞系 (肺腺癌细胞、乳腺癌细胞和神经胶质瘤细胞) 存在变化的情况下。纳米颗粒的表面用聚乙二醇衍生的磷脂进行修饰，以增强生物相容性、水溶性和在水介质中的稳定性。虽然在最高浓度 500μg/ml 下孵育 18 小时时，细胞毒性可以忽略不计，但 MnO 纳米颗粒在孵育 2 和 4 天后的毒性比 Fe(3)O(4)和商业医学造影剂 Feridex 更高。然而，基于活/死细胞活力检测，Fe(3)O(4)的细胞毒性与 Feridex 相当或更好。与 Feridex 相比，经过长时间孵育，工程化的 MnO 和 Fe(3)O(4)表现出优异的稳定性。
