S Jafari, M Cheki, M B Tavakoli, A Zarrabi, K Ghazikhanlu Sani, R Afzalipour
PhD, Department of Radiology Technology, School of Paramedicine, Hamadan University of Medical Sciences, Hamadan, Iran.
PhD, Department of Radiologic Technology, Faculty of Paramedicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Biomed Phys Eng. 2020 Feb 1;10(1):15-24. doi: 10.31661/jbpe.v0i0.929. eCollection 2020 Feb.
Radiosensitization using nanoparticles is proposed as a novel strategy for treatment of different cancers. Superparamagnetic iron oxide nanoparticles (SPIONs) have been reported to enhance effects of radiotherapy in several researches.
The objective of this research is to investigate the radiosensitization properties of polyglycerol coated SPIONs (PG-SPIONs) on U87-MG cancer cells.
In this experimental study, polyglycerol coated SPIONs were synthesized by thermal decomposition method and characterized by FTIR, TEM and VSM analysis. Cellular uptake of nanoparticles by cells was examined via AAS. Cytotoxicity and radiosensitization of nanoparticles in combination with radiation were evaluated by MTT and colony assay, respectively.
Mean size of nanoparticles was 17.9±2.85 nm. FTIR verified SPIONs coating by Polyglycerol and VSM showed that they have superparamagnetic behaviour. Viability significantly (P < 0.001) decreased at concentrations above 100µg/ml for SPIONs but not for PG-SPIONs (P > 0.05). Dose verification results by TLD for doses of 2 and 4 Gy were 2±0.19 and 4±0.12 Gy respectively. The combination index for all situations was less than 1 and the effect is antagonism.
However, PG-SPIONs combination with 6 MV X-ray reduced survival of U87-MG cells compared to radiation alone but the effect is antagonism.
使用纳米颗粒进行放射增敏被认为是治疗不同癌症的一种新策略。在多项研究中，超顺磁性氧化铁纳米颗粒（SPIONs）已被报道可增强放射治疗效果。
本研究旨在探讨聚甘油包被的SPIONs（PG-SPIONs）对U87-MG癌细胞的放射增敏特性。
在本实验研究中，通过热分解法合成聚甘油包被的SPIONs，并通过傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）和振动样品磁强计（VSM）分析对其进行表征。通过原子吸收光谱法（AAS）检测细胞对纳米颗粒的摄取。分别通过MTT法和集落形成试验评估纳米颗粒与辐射联合作用时的细胞毒性和放射增敏作用。
纳米颗粒的平均粒径为17.9±2.85 nm。FTIR证实了聚甘油对SPIONs的包被，VSM显示它们具有超顺磁性行为。当浓度高于100μg/ml时，SPIONs可使细胞活力显著降低（P < 0.001），而PG-SPIONs则无此作用（P > 0.05）。热释光剂量仪（TLD）对2 Gy和4 Gy剂量的剂量验证结果分别为2±0.19 Gy和4±0.12 Gy。所有情况下的联合指数均小于1，其作用为拮抗作用。
然而，与单纯放疗相比，PG-SPIONs与6 MV X射线联合使用可降低U87-MG细胞的存活率，但其作用为拮抗作用。
