Feng Yan, Gu Ya-Lan, Nie Min-Hai, Zhang Qi-Mei, Liang Shang-Zheng
Dept. of Oral Medicine, The Affiliated Dental Hospital of Luzhou Medical College, Luzhou 646000, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2009 Jun;27(3):241-3, 247.
To elucidate the possible mechanism of oral carcinogenesis and to explore the value of clinical application of the detection of cytokeratin (CK) 19 for oral squamous cell carcinoma (OSCC) patients.
The cancerous tissues, para-cancerous tissues and excised lymph nodes were collected from 20 operated patients with OSCC. The patients didn't receive radiotherapy and chemotherapy before hospitalization. The relative expression of CK19 mRNA in those tissues was detected by fluorescent quantitative polymerase chain reaction (FQ-PCR).
The expression of CK19 mRNA in the cancerous tissues was 1.85 and 1.66 times higher than that in normal oral mucosa and in para-cancerous tissues, respectively. The expression of CK19 mRNA in lymph nodes from 9 patients with OSCC was positive and the positive rate was 45% (9/20). The positive rate of CK19 mRNA in all lymph nodes from 9 patients with OSCC was 81.8% (18/22), and the positive rate of CK19 mRNA in all lymph nodes from 20 patients with OSCC was 41.9%(18/43). CK19 mRNA level in the cancerous tissues relative to para-cancerous tissues and normal oral mucosa of the patients whose CK19 mRNA expression was positive was lower than that of the patients whose CK19 mRNA expression was negative in lymph nodes, respectively.
The possible reason that the expression of CK19 mRNA in the cancerous tissues was higher than that in para-cancerous tissues and normal oral mucosa was that the CK19 synthesis in cancerous tissues increased obviously. The detection of CK19 mRNA in lymph nodes was regarded probably as one of the markers for detecting OSCC micrometastasis in lymph nodes. The detection of CK19 mRNA in lymph nodes by FQ-PCR was more sensitive than hematoxylin-eosin staining in diagnosing OSCC micrometastasis.
阐明口腔癌发生的可能机制，并探讨细胞角蛋白（CK）19检测在口腔鳞状细胞癌（OSCC）患者临床应用中的价值。
收集20例接受手术治疗的OSCC患者的癌组织、癌旁组织及切除的淋巴结。患者入院前未接受放疗和化疗。采用荧光定量聚合酶链反应（FQ-PCR）检测这些组织中CK19 mRNA的相对表达量。
癌组织中CK19 mRNA的表达分别比正常口腔黏膜和癌旁组织高1.85倍和1.66倍。9例OSCC患者淋巴结中CK19 mRNA表达呈阳性，阳性率为45%（9/20）。9例OSCC患者所有淋巴结中CK19 mRNA的阳性率为81.8%（18/22），20例OSCC患者所有淋巴结中CK19 mRNA的阳性率为41.9%（18/43）。CK19 mRNA表达阳性患者的癌组织中CK19 mRNA水平相对于癌旁组织和正常口腔黏膜，分别低于CK19 mRNA表达阴性患者淋巴结中的水平。
癌组织中CK19 mRNA表达高于癌旁组织和正常口腔黏膜的可能原因是癌组织中CK19合成明显增加。淋巴结中CK19 mRNA的检测可能被视为检测OSCC淋巴结微转移的标志物之一。FQ-PCR检测淋巴结中CK19 mRNA在诊断OSCC微转移方面比苏木精-伊红染色更敏感。
