Tan Dan, Li Chun-Hui, Nie Min-Hai
Department of Oral Medicine, Hospital of Stomatology, Luzhou Medical College. Luzhou 646000, Sichuan Province, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2016 Oct;25(5):600-603.
To investigate the expression of cytokeratin 19 (CK19) in various stages of oral squamous cell carcinoma (OSCC), and to explore the relation between CK19 and OSCC.
Forty-nine specimens including normal oral mucosa, epithelial hyperplasia, epithelial dysplasia, and oral squamous carcinoma were investigated. The expression of CK19 was detected by immunohistochemistry and Western blot. The serum of OSCC patients and healthy people was collected and CYFRA21-1 level was determined by ELISA. SPSS17.0 software package was used for data elevated.
CK19 was detectable in suprabasal cell layers in epithelia dysplasia and in oral cancer, especially in poorly-differentiated cancerous cells. With epithelia dysplasia becoming worse, the positive rate and the intensity of CKI9 raised significantly. CYKA21-1 in OSCC was significantly higher than that in normal control group(P<0.01).
CK19 overexpression is an early event in the process of oral mucosal canceration. Its abnormal expression can be used as one of the reliable indexes of early diagnosis of OSCC.
研究细胞角蛋白19（CK19）在口腔鳞状细胞癌（OSCC）各阶段的表达情况，并探讨CK19与OSCC之间的关系。
对49份标本进行研究，包括正常口腔黏膜、上皮增生、上皮发育异常及口腔鳞状细胞癌标本。采用免疫组织化学和蛋白质印迹法检测CK19的表达。收集OSCC患者和健康人的血清，采用酶联免疫吸附测定法（ELISA）检测CYFRA21-1水平。使用SPSS17.0软件包进行数据统计分析。
在发育异常上皮和口腔癌的基底上层细胞中可检测到CK19，尤其在低分化癌细胞中。随着上皮发育异常程度加重，CK19的阳性率和表达强度显著升高。OSCC患者血清CYFRA21-1水平显著高于正常对照组（P<0.01）。
CK19过表达是口腔黏膜癌变过程中的早期事件。其异常表达可作为OSCC早期诊断的可靠指标之一。
