Shi R Z, Seeley E S, Bowen R, Faix J D
Department of Pathology, Stanford University, Stanford, California, USA.
J Clin Pathol. 2009 Aug;62(8):752-3. doi: 10.1136/jcp.2008.062547.
To determine whether tubes containing sodium fluoride negatively bias blood glucose concentration by directly comparing glucose concentrations in paired blood samples collected in tubes containing lithium heparin (Li-Heparin) and tubes containing sodium fluoride/potassium oxalate (NaF-KOx).
Paired blood samples from a group of patients (n = 1040) were collected in tubes containing Li-Heparin and tubes containing NaF-KOx at the same time. All Li-Heparin samples were centrifuged soon after collection and were kept cool in transport along with NaF-KOx samples, which were centrifuged at the receiving location after an average transport time of 4 h, but immediately before analysis. Glucose concentrations in the paired samples were determined simultaneously by an automated oxidase method.
The mean glucose concentrations for NaF-KOx samples and Li-Heparin samples were 5.7 mmol/l and 6.1 mmol/l, respectively, with a mean difference of 0.39 mmol/l.
Rapid separation of heparinised blood is superior to fluoride alone for abrogating glycolytic effects on blood glucose measurements in the clinical laboratory.
通过直接比较在含锂肝素（Li - 肝素）的试管和含氟化钠/草酸钾（NaF - KOx）的试管中采集的配对血样中的葡萄糖浓度，来确定含氟化钠的试管是否会对血糖浓度产生负偏差。
同时从一组患者（n = 1040）中采集配对血样，分别置于含Li - 肝素的试管和含NaF - KOx的试管中。所有Li - 肝素样本在采集后不久即进行离心，并在运输过程中与NaF - KOx样本一起冷藏，NaF - KOx样本在平均运输4小时后于接收地点离心，但在分析前立即离心。通过自动氧化酶法同时测定配对样本中的葡萄糖浓度。
NaF - KOx样本和Li - 肝素样本的平均葡萄糖浓度分别为5.7 mmol/l和6.1 mmol/l，平均差值为0.39 mmol/l。
在临床实验室中，对于消除糖酵解对血糖测量的影响，快速分离肝素化血液优于单独使用氟化物。
