le Roux C W, Wilkinson S D, Pavitt D V, Muller B R, Alaghband-Zadeh J
Department of Chemical Pathology, Charing Cross Hospital, London W6 8RF, UK.
Ann Clin Biochem. 2004 Jan;41(Pt 1):43-6. doi: 10.1258/000456304322664681.
Glycolysis is not completely or predictably inhibited by the glucose preservative currently in use, with glucose values falling by as much as 0.5 mmol/L during a 2-4-h period after sample collection. Immediate centrifugation of all samples is also impractical and therefore misdiagnosis of disease can occur, especially if more emphasis is being placed on fasting glucose for the diagnosis of diabetes.
Glycolysis at room temperature was evaluated over time using glyceraldehyde alone as well as in conjunction with standard antiglycolytic agents.
Glyceraldehyde alone does not inhibit glycolysis completely. The combination of 11 mmol/L glyceraldehyde, 119 mmol/L sodium fluoride and 21.7 mmol/L potassium oxalate gave the best antiglycolytic results. The glucose values measured in samples stored at room temperature for 48 h was no different from those measured in samples centrifuged immediately after venepuncture and this is clinically superior to conventionally used sodium fluoride and potassium oxalate.
Plasma glucose concentrations obtained from blood collected into tubes containing glyceraldehyde, sodium fluoride and potassium oxalate will more closely reflect those of the patient at venepuncture.
目前使用的葡萄糖防腐剂并不能完全或可预测地抑制糖酵解，样本采集后2 - 4小时内葡萄糖值可下降多达0.5 mmol/L。对所有样本立即进行离心也不切实际，因此可能会出现疾病误诊，尤其是在糖尿病诊断更侧重于空腹血糖的情况下。
使用甘油醛单独以及与标准抗糖酵解剂联合使用，对室温下的糖酵解随时间进行评估。
单独使用甘油醛不能完全抑制糖酵解。11 mmol/L甘油醛、119 mmol/L氟化钠和21.7 mmol/L草酸钾的组合产生了最佳的抗糖酵解效果。在室温下储存48小时的样本中测得的葡萄糖值与静脉穿刺后立即离心的样本中测得的葡萄糖值没有差异，这在临床上优于传统使用的氟化钠和草酸钾。
从采集到含有甘油醛、氟化钠和草酸钾的试管中的血液获得的血浆葡萄糖浓度将更紧密地反映静脉穿刺时患者的血糖浓度。
