Kumano Y, Yamamoto M, Inomata H, Sakuma S, Hidaka Y, Minagawa H, Mori R
Department of Ophthalmology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Ophthalmologica. 1990;201(4):169-73. doi: 10.1159/000310147.
A 35-year-old man had developed recurrent herpetic keratitis characterized by dendritic keratitis at intervals of a year. We were able to culture cytopathic agents repeatedly from his lesions by inoculating Vero cells. The cultures yielded definitive evidence of a virus that caused a cytopathic effect within 3 days. However, these virus strains could not be identified as herpes simplex virus (HSV) in immunofluorescence assays using the Syva MicroTrak HSV1/HSV2 direct specimen identification/typing test. Rather they were identified as strains of HSV type 1 (HSV-1) on the basis of plaque morphology, neutralization tests, electron-microscopic examination and DNA restriction endonuclease analysis. Our results allow us to assume the existence of HSV-1 strains isolated clinically that are negative to analysis using the Syva Micro-Trak HSV1/HSV2 direct specimen identification/typing test.
一名35岁男性反复发生疱疹性角膜炎，表现为每年发作一次的树枝状角膜炎。通过接种Vero细胞，我们能够从他的病变部位反复培养出细胞病变因子。培养物产生了明确的证据，证明有一种病毒在3天内引起了细胞病变效应。然而，在使用Syva MicroTrak HSV1/HSV2直接标本鉴定/分型试验的免疫荧光测定中，这些病毒株不能被鉴定为单纯疱疹病毒（HSV）。相反，根据噬斑形态、中和试验、电子显微镜检查和DNA限制性内切酶分析，它们被鉴定为1型HSV（HSV-1）株。我们的结果使我们能够假定临床上分离出的HSV-1株在用Syva Micro-Trak HSV1/HSV2直接标本鉴定/分型试验进行分析时呈阴性。
