Lipson S M, Schutzbank T E, Szabo K
J Clin Microbiol. 1987 Feb;25(2):391-4. doi: 10.1128/jcm.25.2.391-394.1987.
Three pairs of monoclonal antibodies, supplied in kits by Electro-Nucleonics, Inc. (ENI), The Syva Co., and Kallestad Laboratories, Inc. (KL), were evaluated for the laboratory confirmation and typing of herpes simplex virus (HSV). Of 108 coded HSV slide preparation, run in parallel with each monoclonal-antibody set, 103 were equivalent by the immunofluorescence assays. Among the five discordant isolates, three (2.8%) did not type with the KL monoclonal antibodies and two (1.9%) false-positive results occurred with the Syva typing system. All of the HSV clinical isolates tested were correctly typed with the ENI indirect immunofluorescence antigen detection system. Typing confirmation of the five discordant HSV isolates was performed by differential sensitivity to 5-bromo-2'-deoxyuridine and endonuclease cleavage analysis of the viral DNA. Use of the Syva and KL direct immunofluorescence antigen detection systems for the identification of HSV isolates is less time-consuming than use of the ENI indirect antigen detection system; however, sensitivity and specificity may be lost.
对由电子核子学公司（ENI）、西瓦公司（The Syva Co.）和卡莱斯塔德实验室公司（KL）试剂盒提供的三对单克隆抗体进行了评估，用于单纯疱疹病毒（HSV）的实验室确诊和分型。在与每套单克隆抗体平行进行检测的108份编码HSV玻片标本中，通过免疫荧光测定法有103份结果一致。在五份不一致的分离株中，三份（2.8%）不能用KL单克隆抗体分型，两份（1.9%）在西瓦分型系统中出现假阳性结果。所有检测的HSV临床分离株都能用ENI间接免疫荧光抗原检测系统正确分型。通过对5-溴-2'-脱氧尿苷的差异敏感性和病毒DNA的核酸内切酶切割分析，对五份不一致的HSV分离株进行了分型确认。使用西瓦和KL直接免疫荧光抗原检测系统鉴定HSV分离株比使用ENI间接抗原检测系统耗时少；然而，可能会损失敏感性和特异性。
