单纯疱疹病毒荧光标记分型试剂的生产。
Production of herpes simplex virus fluorescein labelled typing reagents.
作者信息
Wilson P, Cropper L M, Patt R, Sharp I R
机构信息
Central Public Health Laboratory, Laboratory of Microbiological Reagents, London, UK.
出版信息
J Virol Methods. 1993 Nov;45(1):19-26. doi: 10.1016/0166-0934(93)90136-f.
29 herpes simplex virus monoclonal antibodies (MAbs) were produced and shown by indirect immunofluorescence assay (IIFA) to be HSV type specific or HSV cross reactive. Two HSV-1 and two HSV-2 specific MAbs were selected as HSV typing reagents on the basis of their specificity and the fluorescence pattern produced. When directly conjugated to fluorescein both reagents detected and correctly identified HSV isolates previously typed by restriction endonuclease analysis (REA). No cross reactions were observed with a panel of human viral pathogens. Assessment of these reagents in parallel with the Syva Microtrak Culture Identification/Typing Test indicated 100% concordance for the detection and typing of 80 clinical isolates.
制备了29种单纯疱疹病毒单克隆抗体（MAbs），通过间接免疫荧光测定法（IIFA）显示其具有单纯疱疹病毒（HSV）型特异性或HSV交叉反应性。基于其特异性和产生的荧光模式，选择了两种HSV-1特异性单克隆抗体和两种HSV-2特异性单克隆抗体作为HSV分型试剂。当直接与荧光素偶联时，两种试剂均能检测并正确鉴定先前通过限制性内切酶分析（REA）分型的HSV分离株。与一组人类病毒病原体未观察到交叉反应。与Syva Microtrak培养鉴定/分型试验并行评估这些试剂表明，对80株临床分离株的检测和分型一致性为100%。
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