柳叶破布木提取物对单纯疱疹病毒1型的抗病毒活性

Antiviral activity of an extract of Cordia salicifolia on herpes simplex virus type 1.

作者信息

Hayashi K, Hayashi T, Morita N, Niwayama S

机构信息

Department of Virology, Toyama Medical and Pharmaceutical University, Toyama, Japan.

出版信息

Planta Med. 1990 Oct;56(5):439-43. doi: 10.1055/s-2006-961006.

DOI:10.1055/s-2006-961006

PMID:1963951

Abstract

A partially purified extract (COL 1-6) from whole plant of Cordia salicifolia showed an inhibitory effect on herpes simplex virus type 1 (HSV-1). The activity of COL 1-6 on different steps of HSV-1 replication in HeLa cells was investigated. Under single-cycle replication conditions, COL 1-6 exerted a greater than 99.9% inhibition in virus yield when added to the cells 3 h or 1.5 h before infection, and even when added 8 h after infection the extract still caused a greater than 99% inhibition. The extract has been shown to have a direct virucidal activity. And also, analysis of early events following infection showed that COL 1-6 affected viral penetration in HeLa cells but did not interfere with adsorption to the cells.

摘要

来自柳叶破布木全株的部分纯化提取物（COL 1-6）对1型单纯疱疹病毒（HSV-1）显示出抑制作用。研究了COL 1-6对HSV-1在HeLa细胞中复制不同阶段的活性。在单周期复制条件下，当在感染前3小时或1.5小时添加到细胞中时，COL 1-6对病毒产量的抑制率大于99.9%，甚至在感染后8小时添加该提取物仍能产生大于99%的抑制作用。该提取物已被证明具有直接杀病毒活性。此外，对感染后早期事件的分析表明，COL 1-6影响HSV-1在HeLa细胞中的穿透，但不干扰其对细胞的吸附。