Debiaggi M, Pagani L, Cereda P M, Landini P, Romero E
Institute of Microbiology, University of Pavia, Italy.
Microbiologica. 1988 Jan;11(1):55-61.
Ethanolic extract of the green part of Chamaecyparis Lawsoniana was tested for antiviral activity and toxicity in tissue culture. All experiments were carried out in confluent human embryo lung fibroblasts. Treatment of fibroblast cells with extracts after viral inoculation was effective in inhibiting the replication of herpes simplex virus type 2 (HSV-2). The critical time for virus inhibition was 4 to 5 h after virus absorption. The antiviral activity was assayed employing the techniques of viral plaque and yield reduction. Toxicity of Chamaecyparis Lawsoniana in uninfected cells was studied as alteration of cell morphology, cellular viability and inhibition of host cell DNA synthesis. Herpes simplex virus inhibition occurred in presence of extract concentration of 0.5 micrograms/ml, whereas concentrations exceeding 5 micrograms/ml and 140 micrograms/ml were found to be cytotoxic when evaluated with inhibition of host DNA synthesis and cellular viability respectively. Results suggest that further investigations concerning the isolation of substances responsible for antiviral activity and an effort to define the mechanisms of action are warranted.
对美国扁柏绿色部分的乙醇提取物进行了组织培养中的抗病毒活性和毒性测试。所有实验均在汇合的人胚肺成纤维细胞中进行。病毒接种后用提取物处理成纤维细胞可有效抑制单纯疱疹病毒2型（HSV-2）的复制。病毒抑制的关键时间是病毒吸附后4至5小时。采用病毒蚀斑和产量降低技术测定抗病毒活性。研究了美国扁柏在未感染细胞中的毒性，观察其对细胞形态、细胞活力和宿主细胞DNA合成的抑制作用。当提取物浓度为0.5微克/毫升时可抑制单纯疱疹病毒，而分别用宿主DNA合成抑制和细胞活力评估时，发现浓度超过5微克/毫升和140微克/毫升具有细胞毒性。结果表明，有必要进一步研究分离负责抗病毒活性的物质，并努力确定其作用机制。
