[不同干燥工艺所得忍冬花蕾的高效液相色谱指纹图谱比较]
[Comparison on HPLC fingerprint profiles of Lonicera japonica bud obtained by various dryness techniques].
作者信息
Xiong Yan, Gao Huimin, Wang Zhiming, Zhu Jingjing
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Apr;34(8):1015-7.
OBJECTIVE
To investigate ingredient differences of Lonicera japonica bud processed by different dryness techniques.
METHOD
The HPLC fingerprint was used to evaluate the quality of L. japonica by various dryness process. The analysis was performed on a Kromasil C18 (4.6 mm x 250 mm, 5 microm). Acetonitril and water containing 0.4% H3PO4 were used as mobile phases with gradient elution. The flow rate was set at 1.0 mL x min(-1), the column temperature was set at 30 degrees C, and the detection wavelength was at 238 nm.
RESULT
The HPLC-fingerprint profiles of L. japonica from six different dryness techniques were determined. A total of 17 common peaks were found, but their contents from various dryness techniques were significantly different.
CONCLUSION
HPLC-fingerprint technique was one of potential Process Analytical Techniques (PAT) to explore the ingredient differences in dryness process.
目的
研究不同干燥工艺处理的忍冬花蕾的成分差异。
方法
采用高效液相色谱指纹图谱法评价不同干燥工艺下忍冬的质量。分析在Kromasil C18柱（4.6 mm×250 mm，5μm）上进行。以含0.4%磷酸的乙腈和水为流动相进行梯度洗脱。流速设定为1.0 mL·min⁻¹，柱温设定为30℃，检测波长为238 nm。
结果
测定了六种不同干燥工艺下忍冬的高效液相色谱指纹图谱。共发现17个共有峰，但不同干燥工艺下它们的含量有显著差异。
结论
高效液相色谱指纹图谱技术是探索干燥过程中成分差异的潜在过程分析技术之一。
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