Vats Purva, Yu Ji, Rothfield Lawrence
Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030, USA.
Cell Mol Life Sci. 2009 Oct;66(20):3353-62. doi: 10.1007/s00018-009-0092-5. Epub 2009 Jul 30.
Three of the four well-established bacterial cytoskeletal systems-the MreB, MinCDE, and FtsZ systems-undergo a variety of short-range and long-range dynamic behaviors. These include the cellular reorganization of the cytoskeletal elements, in which the proteins redistribute from a predominantly helical pole-to-pole pattern into annular structures near midcell. Despite their apparent similarity, these dramatic redistributional events in the three systems are in large part independent of each other. In addition, some of the cytoskeletal structures undergo oscillatory behavior in which the helical elements move repetitively back-and-forth between the two ends of the cell. The details and mechanisms underlying these dynamic cellular events are just now being revealed by fluorescence microscopy of intact cells, fluorescence photobleaching recovery studies, single molecule tracking techniques, and in vitro studies of the purified proteins.
四个成熟的细菌细胞骨架系统中的三个——MreB、MinCDE和FtsZ系统——会经历各种短程和长程动态行为。这些行为包括细胞骨架元件的细胞重组，其中蛋白质从主要的螺旋状极到极模式重新分布到细胞中部附近的环形结构中。尽管它们表面相似，但这三个系统中这些显著的重新分布事件在很大程度上是相互独立的。此外，一些细胞骨架结构会发生振荡行为，其中螺旋元件在细胞的两端之间反复来回移动。这些动态细胞事件背后的细节和机制刚刚通过完整细胞的荧光显微镜、荧光光漂白恢复研究、单分子追踪技术以及纯化蛋白质的体外研究得以揭示。
