Eichhorst Jeff C, Etter Michele L, Rousseaux Nadine, Lehotay Denis C
Saskatchewan Disease Control Laboratory, SK, Canada.
Clin Biochem. 2009 Oct;42(15):1531-42. doi: 10.1016/j.clinbiochem.2009.07.019. Epub 2009 Jul 28.
To replace immunoassay screening for drugs of abuse (DOA) with a cost-effective tandem mass spectrometry method.
To substantially expand the drugs of abuse assay menu.
The requirement was to perform high throughput DOA screening for 200 urine specimens/day for 40 drugs/metabolites. The total analysis time had to be <5 min. We used UPLC chromatography, small particle size LC columns and fast scanning tandem mass spectrometry. Urine samples were hydrolyzed enzymatically, diluted and injected with isotopically labeled internal standards. The data produced was transferred by exporting reports as text files to a LIMS system followed by auto certification of the results.
40 different drugs were separated by UPLC (ultra pressure liquid chromatography) with a run time of 5.2 min. Detection limits were below our cut-off values. Individual drug species instead of drug classes were identified; correlation with GC/MS was excellent. A high throughput, robust assay with acceptable accuracy, precision and specificity was developed. The procedure can also be used as a quantitative method with simple modifications.
An improved, high throughput, cost-effective method for drugs of abuse screening has been implemented. GC/MS confirmations were reduced or eliminated. The new procedure is a viable alternative to our previous immunoassay method. Acceptable turn around times, an expanded menu, simplified sample preparation and analytical reliability makes this method a desirable option in the clinical laboratory setting.
用一种具有成本效益的串联质谱法取代对滥用药物（DOA）的免疫分析筛查。
大幅扩展滥用药物分析项目。
要求每天对200份尿液标本进行40种药物/代谢物的高通量DOA筛查。总分析时间必须<5分钟。我们使用超高效液相色谱（UPLC）、小粒径液相色谱柱和快速扫描串联质谱。尿液样本经酶解、稀释并注入同位素标记的内标。产生的数据通过将报告导出为文本文件传输到实验室信息管理系统（LIMS），随后自动认证结果。
通过超高效液相色谱（UPLC）分离出40种不同的药物，运行时间为5.2分钟。检测限低于我们的临界值。识别出的是单个药物种类而非药物类别；与气相色谱/质谱（GC/MS）的相关性极佳。开发出了一种具有可接受的准确度、精密度和特异性的高通量、稳健的分析方法。该程序经简单修改后也可用作定量方法。
已实施一种改进的、高通量、具有成本效益的滥用药物筛查方法。气相色谱/质谱（GC/MS）确证减少或消除。新程序是我们之前免疫分析方法的可行替代方法。可接受的周转时间、扩展的项目、简化的样本制备和分析可靠性使该方法成为临床实验室环境中理想的选择。
