Department of Chemistry, University of Wisconsin-La Crosse, La Crosse, WI 54601, USA.
Planta Med. 2010 Feb;76(2):182-5. doi: 10.1055/s-0029-1186001. Epub 2009 Jul 30.
Antibacterial bioassay-guided fractionation of two American mushroom species, Jahnoporus hirtus and Albatrellus flettii, led to the isolation and identification of their major antibacterial constituents: 3,11-dioxolanosta-8,24( Z)-diene-26-oic acid (1) from J. hirtus and confluentin (2), grifolin (3), and neogrifolin (4) from A. flettii. Compound 1 is a new lanostane-type triterpene. All purified compounds were evaluated for their ability to inhibit the growth of Bacillus cereus and Enterococcus faecalis using standard MIC assays. Compounds 1- 4 demonstrated MIC values of 40, 20, 10, and 20 microg/mL, respectively, against B. cereus and MIC values of 32, 1.0, 0.5, and 0.5 microg/mL, respectively, against E. faecalis. Thus, one novel compound and three others were shown to possess antimicrobial activities against these gram-positive bacteria employed as surrogates for more virulent and dangerous pathogens.
抗菌生物测定指导下的两种美国蘑菇物种 Jahnoporus hirtus 和 Albatrellus flettii 的分离，导致了它们主要抗菌成分的分离和鉴定：来自 J. hirtus 的 3,11-二氧杂甾烷-8,24(Z)-二烯-26-酸（1）和来自 A. flettii 的 confluentin（2）、grifolin（3）和 neogrifolin（4）。化合物 1 是一种新的羊毛甾烷型三萜。所有纯化的化合物均通过标准 MIC 测定法评估其抑制蜡状芽孢杆菌和粪肠球菌生长的能力。化合物 1-4 对蜡状芽孢杆菌的 MIC 值分别为 40、20、10 和 20 μg/mL，对粪肠球菌的 MIC 值分别为 32、1.0、0.5 和 0.5 μg/mL。因此，一种新化合物和另外三种化合物显示出对这些革兰氏阳性菌具有抗菌活性，这些菌被用作更具毒性和危险病原体的替代物。
