Department of Chemistry, University of Wisconsin - La Crosse, La Crosse, Wisconsin 54601, USA.
Planta Med. 2010 Mar;76(5):464-6. doi: 10.1055/s-0029-1186227. Epub 2009 Oct 21.
Anti- Bacillus cereus bioassay-guided fractionation of a crude extract of the American mushroom, Fomitopsis pinicola, was performed using thin-layer chromatography, Sephadex LH-20 column chromatography, and preparative-scale HPLC. Five lanostane triterpenoids (1-5) and one ergostane steroid (6) were isolated and identified. Compound 1 is a new lanostane triterpenoid, and its structure was determined using 1D and 2D NMR experiments, HR-MS, and physical data. Each of the purified compounds (1-6) was tested for antibacterial activity against B. cereus using standard MIC assays. Compounds 1-6 had MIC values of 32, 16, 32, 32, 128, and 64 microg/mL, respectively.
采用薄层层析、葡聚糖 LH-20 柱层析和制备高效液相色谱法对美国蘑菇 Fomitopsis pinicola 的粗提取物进行抗蜡状芽孢杆菌生物测定导向分离。分离并鉴定了五种羊毛甾烷三萜（1-5）和一种麦角甾烷甾体（6）。化合物 1 是一种新的羊毛甾烷三萜，其结构通过 1D 和 2D NMR 实验、高分辨率质谱和物理数据确定。用标准 MIC 测定法测试了每种纯化化合物（1-6）对蜡状芽孢杆菌的抗菌活性。化合物 1-6 的 MIC 值分别为 32、16、32、32、128 和 64μg/mL。
