来自英国一株传染性胃肠炎病毒强毒株的S基因序列。
Sequence of the S gene from a virulent British field isolate of transmissible gastroenteritis virus.
作者信息
Britton P, Page K W
机构信息
A.F.R.C., Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, U.K.
出版信息
Virus Res. 1990 Dec;18(1):71-80. doi: 10.1016/0168-1702(90)90090-x.
Abstract
Subgenomic mRNA from a virulent field isolate of porcine transmissible gastroenteritis virus (TGEV), strain FS772/70, was used to produce cDNA. The cDNA from three overlapping clones was sequenced by the chain termination method and two open reading frames (ORFs) were identified. The largest ORF, 4350bp, encoded a polypeptide of 1449 amino acids of relative molecular mass (Mr) 159811, which contained 33 potential N-linked glycosylation sites, a cysteine-rich region, and a potential transmembrane region. The C-terminal half of this ORF showed homology to the S proteins of four other coronaviruses. The other ORF consisted of the 3'-end of a gene with homology to the carboxyl terminus of the F2 subunit of infectious bronchitis virus (IBV) RNA polymerase.
摘要
来自猪传染性胃肠炎病毒（TGEV）强毒株FS772/70的亚基因组mRNA被用于制备cDNA。通过链终止法对来自三个重叠克隆的cDNA进行测序，并鉴定出两个开放阅读框（ORF）。最大的ORF为4350bp，编码一个含有1449个氨基酸、相对分子质量（Mr）为159811的多肽，该多肽含有33个潜在的N-连接糖基化位点、一个富含半胱氨酸的区域和一个潜在的跨膜区域。该ORF的C端一半与其他四种冠状病毒的S蛋白具有同源性。另一个ORF由一个与传染性支气管炎病毒（IBV）RNA聚合酶F2亚基羧基末端具有同源性的基因的3'端组成。
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