Belderbos M E, van Bleek G M, Levy O, Blanken M O, Houben M L, Schuijff L, Kimpen J L L, Bont L
Wilhelmina Children's Hospital, University Medical Centre Utrecht, Utrecht, The Netherlands.
Clin Immunol. 2009 Nov;133(2):228-37. doi: 10.1016/j.clim.2009.07.003. Epub 2009 Aug 3.
Newborns are highly susceptible to infectious diseases, which may be due to impaired immune responses. This study aims to characterize the ontogeny of neonatal TLR-based innate immunity during the first month of life. Cellularity and Toll-like receptor (TLR) agonist-induced cytokine production were compared between cord blood obtained from healthy neonates born after uncomplicated gestation and delivery (n=18), neonatal venous blood obtained at the age of one month (n=96), and adult venous blood (n=17). Cord blood TLR agonist-induced production of the Th1-polarizing cytokines IL-12p70 and IFN-alpha was generally impaired, but for TLR3, 7 and 9 agonists, rapidly increased to adult levels during the first month of life. In contrast, TLR4 demonstrated a slower maturation, with low LPS-induced IL-12p70 production and high IL-10 production up until the age of one month. Polarization in neonatal cytokine responses to LPS could contribute to neonatal susceptibility to severe bacterial infection.
新生儿极易感染传染病，这可能是由于免疫反应受损所致。本研究旨在描述出生后第一个月内基于新生儿Toll样受体（TLR）的先天免疫的个体发生情况。比较了来自妊娠和分娩正常的健康新生儿的脐带血（n = 18）、1月龄新生儿静脉血（n = 96）和成人静脉血（n = 17）的细胞数量以及Toll样受体（TLR）激动剂诱导的细胞因子产生情况。脐带血TLR激动剂诱导的Th1极化细胞因子IL-12p70和IFN-α的产生通常受损，但对于TLR3、7和9激动剂，在出生后的第一个月内迅速增加至成人水平。相比之下，TLR4的成熟较慢，直到1月龄时，脂多糖（LPS）诱导的IL-12p70产生较低，而IL-10产生较高。新生儿对LPS的细胞因子反应极化可能导致新生儿对严重细菌感染易感。
