Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):191-195. doi: 10.1099/ijs.0.011270-0. Epub 2009 Jul 31.
A Gram-negative, motile and pleomorphic bacterial strain, SMK-146(T), was isolated from a tidal flat sediment of the Yellow Sea, Korea, and its taxonomic position was investigated. Strain SMK-146(T) grew optimally at pH 7.0-8.0 and 30 degrees C. It contained Q-10 as the predominant ubiquinone and C(18 : 1)omega7c and 11-methyl C(18 : 1)omega7c as the major fatty acids. The major polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 68.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SMK-146(T) belongs to the genus Jannaschia. Strain SMK-146(T) exhibited 16S rRNA gene sequence similarity values of 95.3-97.0 % to the type strains of the five recognized Jannaschia species. The mean DNA-DNA relatedness value between strain SMK-146(T) and Jannaschia seosinensis KCCM 42114(T), the closest phylogenetic neighbour, was 17 %. Differential phenotypic properties also revealed that strain SMK-146(T) differs from the recognized Jannaschia species. On the basis of phenotypic, phylogenetic and genetic data, strain SMK-146(T) represents a novel species of the genus Jannaschia, for which the name Jannaschia seohaensis sp. nov. is proposed. The type strain is SMK-146(T) (=KCTC 22172(T) =CCUG 55326(T)).
一株革兰氏阴性、运动性和多形性的细菌菌株 SMK-146(T) 从韩国黄海潮滩沉积物中分离得到，并对其分类地位进行了研究。菌株 SMK-146(T) 在 pH7.0-8.0 和 30°C 下生长最佳。它含有 Q-10 作为主要的泛醌，以及 C(18:1)omega7c 和 11-甲基 C(18:1)omega7c 作为主要的脂肪酸。主要的极性脂是磷脂酰胆碱、磷脂酰甘油和磷脂酰乙醇胺。DNA G+C 含量为 68.4mol%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 SMK-146(T) 属于詹纳斯氏菌属。菌株 SMK-146(T) 与 5 种公认的詹纳斯氏菌种的模式株的 16S rRNA 基因序列相似性值为 95.3-97.0%。菌株 SMK-146(T)与最接近的系统发育近邻詹纳斯氏菌 seosinensis KCCM 42114(T)之间的平均 DNA-DNA 同源性值为 17%。差异表型特性也表明，菌株 SMK-146(T)与公认的詹纳斯氏菌种不同。基于表型、系统发育和遗传数据，菌株 SMK-146(T)代表詹纳斯氏菌属的一个新种，命名为詹纳斯氏菌 seohaensis sp. nov.。模式菌株为 SMK-146(T)=KCTC 22172(T)=CCUG 55326(T)。
