Yoon Jaewoo, Oh Ji Hoon
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-gu, Daegu, 42601, Republic of Korea.
Department of Biological Sciences, College of Natural Sciences, Keimyung University, 1095 Dalgubeoldaero, Dalseo-gu, Daegu, 42601, Republic of Korea.
Curr Microbiol. 2025 Jul 2;82(8):358. doi: 10.1007/s00284-025-04331-w.
Phenotypic, physiological, phylogenetic, genomic, and chemotaxonomic analyses were carried out on a bacterium, designated as KMU-145, isolated from seawater gathered in the Republic of Korea. Strain KMU-145 was ovoid-shaped, Gram-reaction-negative, non-motile, obligately aerobic, and formed apricot-colored colonies on marine agar 2216. The strain grew at 15-37 °C, pH 6.0-9.5, and 0-2.0% NaCl concentrations (w/v). Preliminary evaluation based on 16S rRNA gene sequence showed that strain KMU-145 was affiliated with the genus Jannaschia, family Roseobacteraceae, showing the greatest sequence similarity (98.4%) to "J. ovalis" GRR-S6-38. The only respiratory quinone was ubiquinone-10, and the main (> 10%) fatty acids were C ω7c, C ω7c 11-methyl, and C. Phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, three unidentified aminolipids, and three unidentified lipids were found as polar lipids. Strain KMU-145 had a 3.5 Mbp genome with a 68.5% G+C content. The pairwise ANI values between the genomes of strain KMU-145 and other species of the genus Jannaschia were 70.9-79.3%. Strain KMU-145 exhibited 21.6% dDDH value with strain "J. ovalis" GRR-S6-38. The genome of strain KMU-145 contained numerous carbohydrate-active enzymes, suggesting a significant potential for the development of enzymes with practical applications in industry. Therefore, the marine strain represents a novel member of the genus Jannaschia, for which the name Jannaschia halovivens sp. nov., is proposed with the type strain KMU-145 (= KCCM 90597 = NBRC 117092).
对从韩国采集的海水中分离出的一株名为KMU - 145的细菌进行了表型、生理、系统发育、基因组和化学分类分析。KMU - 145菌株呈卵形，革兰氏阴性，无运动性，专性需氧，在海洋琼脂2216上形成杏色菌落。该菌株在15 - 37°C、pH 6.0 - 9.5和0 - 2.0%（w/v）NaCl浓度下生长。基于16S rRNA基因序列的初步评估表明，KMU - 145菌株隶属于玫瑰杆菌科詹氏菌属，与“卵形詹氏菌”GRR - S6 - 38的序列相似性最高（98.4%）。唯一的呼吸醌是泛醌 - 10，主要（>10%）脂肪酸为C ω7c、C ω7c 11 - 甲基和C。磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、三种未鉴定的氨基脂质和三种未鉴定的脂质被发现为极性脂质。KMU - 145菌株的基因组大小为3.5 Mbp，G + C含量为68.5%。KMU - 145菌株与詹氏菌属其他物种基因组之间的成对ANI值为70.9 - 79.3%。KMU - 145菌株与“卵形詹氏菌”GRR - S6 - 38菌株的dDDH值为21.6%。KMU - 145菌株的基因组包含许多碳水化合物活性酶，表明其在工业上开发具有实际应用价值的酶具有巨大潜力。因此，该海洋菌株代表了詹氏菌属的一个新成员，为此提出新名称嗜盐詹氏菌（Jannaschia halovivens sp. nov.），模式菌株为KMU - 145（=KCCM 90597 = NBRC 117092）。
