Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):223-228. doi: 10.1099/ijs.0.010710-0. Epub 2009 Jul 31.
Four strains isolated from cultured Manila clam, Ruditapes philippinarum, in the north-western coast of Spain were characterized phenotypically and genotypically. Phylogenetic analyses based on the 16S rRNA gene sequences indicated that these bacteria were closely related to Aliivibrio wodanis, Aliivibrio salmonicida, Aliivibrio fischeri and Aliivibrio logei with sequence similarities between 98.1 and 96.0 %. Phylogenetic analysis based on the RNA polymerase alpha chain (rpoA), RecA protein (recA), the alpha-subunit of bacterial ATP synthase (atpA) and the uridine monophosphate (UMP) kinase (pyrH) genes and fluorescent amplified fragment length polymorphism experiments clearly showed that these novel isolates form a tight genomic group different from any currently known Aliivibrio species. On the basis of phylogenetic analysis and phenotypic data, the four strains represent a novel taxon, for which the name Aliivibrio finisterrensis sp. nov. is proposed. Several phenotypic features were revealed that discriminate A. finisterrensis from other Aliivibrio species. The type strain is CMJ 11.1(T) (=CECT 7228(T)=LMG 23869(T)).
从西班牙西北部沿海养殖的菲律宾蛤仔中分离出的 4 株菌，进行了表型和基因型特征分析。基于 16S rRNA 基因序列的系统发育分析表明，这些细菌与沃氏拟杆菌、鲑鱼气单胞菌、发光拟杆菌和 Logei 拟杆菌密切相关，序列相似度在 98.1%至 96.0%之间。基于 RNA 聚合酶α链（rpoA）、RecA 蛋白（recA）、细菌三磷酸腺苷合酶α亚基（atpA）和尿苷单磷酸（UMP）激酶（pyrH）基因以及荧光扩增片段长度多态性实验的系统发育分析清楚地表明，这些新分离株形成了一个与任何已知的 Aliivibrio 物种都不同的紧密基因组群。基于系统发育分析和表型数据，这 4 株菌代表了一个新的分类群，建议将其命名为 Aliivibrio finisterrensis sp. nov.。一些表型特征表明，A. finisterrensis 与其他 Aliivibrio 物种不同。其模式菌株为 CMJ 11.1(T)（=CECT 7228(T)=LMG 23869(T)）。
