Klemetsen Terje, Karlsen Christian R, Willassen Nils P
Department of Chemistry, Center for Bioinformatics, UiT The Arctic University of Norway, Tromsø, Norway.
Department of Fish Health, Nofima, Aas, Norway.
Front Microbiol. 2021 Feb 18;12:626759. doi: 10.3389/fmicb.2021.626759. eCollection 2021.
Genus is known to harbor species exhibiting bioluminescence as well as pathogenic behavior affecting the fish farming industry. Current phylogenetic understanding of has largely remained dormant after reclassification disentangled it from the genus in 2007. There is growing evidence of wider diversity, but until now the lack of genomes and selective use of type strains have limited the ability to compare and classify strains firmly. In this study, a total of 143 bacterial strains, including 51 novel sequenced strains, were used to strengthen phylogenetic relationships in by exploring intra-species and inter-species relations. Multilocus sequence analysis (MLSA), applying the six housekeeping genes (), , , , , and , inferred 12 clades and a singular branch in . Along with four new phylogenetic clades, the MLSA resolved prior inconsistencies circumscribing and formed a unique clade we propose as the novel species sp. "friggae." Furthermore, phylogenetic assessment of individual marker genes showed , , and superior to the 16S rRNA gene, resolving accurately for most species clades in . In this study, we provide a robust phylogenetic groundwork for as a reference point to classification of species.
已知该属包含表现出发光以及影响养鱼业的致病行为的物种。在2007年重新分类将其与另一属分离后，目前对该属的系统发育理解在很大程度上一直处于停滞状态。越来越多的证据表明其具有更广泛的多样性，但直到现在，基因组的缺乏和对模式菌株的选择性使用限制了对菌株进行可靠比较和分类的能力。在本研究中，总共143株细菌菌株，包括51株新测序菌株，通过探索种内和种间关系来加强该属内的系统发育关系。多位点序列分析（MLSA）应用六个管家基因（基因名称）推断出该属内有12个进化枝和一个单独的分支。除了四个新的系统发育进化枝外，MLSA解决了之前关于该属界定的不一致问题，并形成了一个独特的进化枝，我们将其提议为新物种“friggae”菌。此外，对单个标记基因的系统发育评估表明，某些基因优于16S rRNA基因，能准确解析该属中大多数物种进化枝。在本研究中，我们为该属提供了一个可靠的系统发育基础，作为物种分类的参考点。
