Unit of Microbiology, Rovira i Virgili University, IISPV, Sant Llorenç 21, 43201, Reus, Spain.
Molecular Diagnostics Center (MDC), Biomolecular Technologies S.L., and Miguel Hernández University, Orihuela E-03300, Alicante. Spain.
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):72-77. doi: 10.1099/ijs.0.011643-0. Epub 2009 Jul 31.
A Gram-stain-negative, facultatively anaerobic bacterial strain, designated 717(T), was isolated from a water sample collected from the Muga river, Girona, north-east Spain. Preliminary analysis of the 16S rRNA gene sequence showed that this strain belonged to the genus Aeromonas, the nearest species being Aeromonas veronii (99.5 % similarity, with seven different nucleotides). A polyphasic study based on a multilocus phylogenetic analysis of five housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 3684 bp) showed isolate 717(T) to be an independent phylogenetic line, with Aeromonas sobria, Aeromonas veronii and Aeromonas allosaccharophila as the closest neighbour species. DNA-DNA reassociation experiments and phenotypic analysis identified that strain 717(T) represents a novel species, for which the name Aeromonas fluvialis sp. nov. is proposed, with type strain 717(T) (=CECT 7401(T) =LMG 24681(T)).
一株革兰氏阴性、兼性厌氧细菌菌株，命名为 717(T)，从西班牙东北部赫罗纳的缪加河采集的水样中分离得到。16S rRNA 基因序列的初步分析表明，该菌株属于气单胞菌属，与气单胞菌 veronii（相似度为 99.5%，有七个不同的核苷酸）最为接近。基于 5 个看家基因（gyrB、rpoD、recA、dnaJ 和 gyrA；3684bp）的多位点系统发育分析的综合研究表明，分离株 717(T)是一个独立的进化分支，与温和气单胞菌、维隆气单胞菌和气单胞菌 allosaccharophila 为最接近的亲缘关系种。DNA-DNA 杂交实验和表型分析表明，菌株 717(T)代表了一个新的物种，建议将其命名为河流气单胞菌新种，其模式菌株为 717(T)=CECT 7401(T)=LMG 24681(T)。
