模块化扫描荧光相关光谱技术可量化活的多细胞生物中的受体-配体相互作用。
Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms.
作者信息
Ries Jonas, Yu Shuizi Rachel, Burkhardt Markus, Brand Michael, Schwille Petra
机构信息
Biotechnology Center, Technical University of Dresden, Dresden, Germany.
出版信息
Nat Methods. 2009 Sep;6(9):643-5. doi: 10.1038/nmeth.1355. Epub 2009 Aug 2.
Analysis of receptor-ligand interactions in vivo is key to biology but poses a considerable challenge to quantitative microscopy. Here we combine static-volume, two-focus and dual-color scanning fluorescence correlation spectroscopy to solve this task at cellular resolution in complex biological environments. We quantified the mobility of fibroblast growth factor receptors Fgfr1 and Fgfr4 in cell membranes of living zebrafish embryos and determined their in vivo binding affinities to their ligand Fgf8.
体内受体 - 配体相互作用的分析是生物学的关键，但对定量显微镜技术提出了相当大的挑战。在这里，我们结合静态体积、双焦点和双色扫描荧光相关光谱技术，在复杂生物环境中以细胞分辨率解决这一任务。我们量化了活斑马鱼胚胎细胞膜中成纤维细胞生长因子受体Fgfr1和Fgfr4的流动性，并确定了它们在体内与配体Fgf8的结合亲和力。
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