恩格勒氏野桐对去势雄性大鼠射精潜能的评估。
Evaluation of the excopula ejaculatory potentials of Bersama engleriana in spinal male rats.
作者信息
Watcho Pierre, Carro-Juarez Miguel
机构信息
Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Dschang, P.O. BOX 67, Cameroon.
出版信息
Asian J Androl. 2009 Sep;11(5):533-9. doi: 10.1038/aja.2009.41. Epub 2009 Aug 3.
The aim of this study was to investigate the effects of Bersama engleriana and its potential mechanism on fictive ejaculation in spinal male rats. The electromyographic activities of the bulbospongiosus muscles were recorded in spinal cord transected and urethane-anesthetized rats treated intravenously with aqueous (100 mg kg(-1)) and methanolic (100 mg kg(-1)) extracts from the dried leaves of B. engleriana in the absence and presence of dopamine (0.1 micromol kg(-1)) or oxytocin (0.5 UI kg(-1)). Mechanical stimulations of the urethra were also carried out 5 min after the sequential treatments. A single intravenous administration of aqueous (100 mg kg(-1)) and methanolic (100 mg kg(-1)) extracts of B. engleriana did not activate fictive ejaculation. The electromyography recorded after the application of the plant extract was similar to that obtained after intravenous saline injection (200 Gl min(-1)) with no contraction of the bulbospongiosus muscles. Dopamine (0.1 micromol kg(-1)) and oxytocin (0.5 UI kg(-1)) induced rapid rhythmic contractions (P < 0.001) of the bulbospongiosus muscles accompanied by penile erection and sometimes with expulsion of the seminal plugs. Pre-treatment of rats with the two plant extracts completely abolished the occurrence of ejaculation induced by dopamine (0.1 micromol kg(-1)) and oxytocin (0.5 UI kg(-1)). Mechanical stimulation of the urethra carried out 5 min after the sequential treatments always induced penile movements and erections. The inhibitory effect of B. engleriana extracts on the expression of fictive ejaculation in spinal male rat is mediated through dopaminergic and oxytocinergic pathways. This prolonged ejaculatory latency caused by B. engleriana could support its potential use in patients with rapid ejaculation.
本研究旨在探讨扁叶布惊对脊髓损伤雄性大鼠假射精的影响及其潜在机制。在脊髓横断并经乌拉坦麻醉的大鼠中，静脉注射扁叶布惊干叶的水提取物（100 mg kg⁻¹）和甲醇提取物（100 mg kg⁻¹），记录球海绵体肌的肌电活动，实验分别在不存在和存在多巴胺（0.1 μmol kg⁻¹）或催产素（0.5 UI kg⁻¹）的情况下进行。在序贯给药后5分钟，还对尿道进行了机械刺激。单次静脉注射扁叶布惊的水提取物（100 mg kg⁻¹）和甲醇提取物（100 mg kg⁻¹）均未激活假射精。应用植物提取物后记录的肌电图与静脉注射生理盐水（200 μl min⁻¹）后相似，球海绵体肌无收缩。多巴胺（0.1 μmol kg⁻¹）和催产素（0.5 UI kg⁻¹）可诱导球海绵体肌快速有节律的收缩（P < 0.001），伴有阴茎勃起，有时还伴有精液栓排出。用两种植物提取物预处理大鼠可完全消除由多巴胺（0.1 μmol kg⁻¹）和催产素（0.5 UI kg⁻¹）诱导的射精。序贯给药后5分钟对尿道进行机械刺激总能诱导阴茎运动和勃起。扁叶布惊提取物对脊髓损伤雄性大鼠假射精表达的抑制作用是通过多巴胺能和催产素能途径介导的。扁叶布惊引起的射精潜伏期延长可能支持其在早泄患者中的潜在应用。
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