使用基于微机电系统扫描镜的便携式2.9克双光子显微镜进行活体脑成像。
In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror.
作者信息
Piyawattanametha Wibool, Cocker Eric D, Burns Laurie D, Barretto Robert P, Jung Juergen C, Ra Hyejun, Solgaard Olav, Schnitzer Mark J
机构信息
James H. Clark Center for Biomedical Engineering and Sciences, Stanford University, Stanford, California 94305, USA.
出版信息
Opt Lett. 2009 Aug 1;34(15):2309-11. doi: 10.1364/ol.34.002309.
Abstract
We present a two-photon microscope that is approximately 2.9 g in mass and 2.0 x 1.9 x 1.1 cm(3) in size and based on a microelectromechanical systems (MEMS) laser-scanning mirror. The microscope has a focusing motor and a micro-optical assembly composed of four gradient refractive index lenses and a dichroic microprism. Fluorescence is captured without the detected emissions reflecting off the MEMS mirror, by use of separate optical fibers for fluorescence collection and delivery of ultrashort excitation pulses. Using this microscope we imaged neocortical microvasculature and tracked the flow of erythrocytes in live mice.
摘要
我们展示了一种双光子显微镜，其质量约为2.9克，尺寸为2.0×1.9×1.1立方厘米，基于微机电系统（MEMS）激光扫描镜。该显微镜有一个聚焦电机和一个由四个梯度折射率透镜和一个二向色微棱镜组成的微光学组件。通过使用单独的光纤进行荧光收集和超短激发脉冲的传输，在检测到的发射光不被MEMS镜反射的情况下捕获荧光。利用这台显微镜，我们对新生小鼠的新皮质微血管进行了成像，并追踪了红细胞的流动情况。
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