Le Harzic R, Weinigel M, Riemann I, König K, Messerschmidt B
Fraunhofer Institute for Biomedical Technology (IBMT), Ensheimer Strasse 48, D-66386 St. Ingbert, Germany.
Opt Express. 2008 Dec 8;16(25):20588-96. doi: 10.1364/oe.16.020588.
We report on a nonlinear optical endoscope that adopts a hollow core photonic crystal fiber for single-mode illumination delivery and a multimode one for signal collection. Femtosecond laser pulses up to 100 mW can be delivered at a centered wavelength of 800 nm. The two-photon fluorescence response of our system is shown to have axial and lateral resolutions of 5.8 microm and 0.6 microm respectively. Fluorescence detection was obtained at different wavelengths between 790 and 840 nm which could allow SHG detection for example. The maximal field-of-view of the acquired images is 420 microm x 420 microm. Detection efficiency is greater by using an avalanche photodiode in comparison to a photo multiplier tube. Results presented here demonstrate the ability of the system to resolve cellular details and the potential of the device for future in vivo imaging diagnosis.
我们报道了一种非线性光学内窥镜，它采用空心光子晶体光纤进行单模照明传输，并采用多模光纤进行信号采集。在中心波长800nm处，可传输高达100mW的飞秒激光脉冲。我们系统的双光子荧光响应显示，轴向分辨率和横向分辨率分别为5.8微米和0.6微米。在790至840nm之间的不同波长处获得了荧光检测结果，例如这可以实现二次谐波产生（SHG）检测。所采集图像的最大视野为420微米×420微米。与光电倍增管相比，使用雪崩光电二极管时检测效率更高。此处展示的结果证明了该系统分辨细胞细节的能力以及该设备在未来体内成像诊断中的潜力。
