Zhuang Yong-liang, Zhao Xue, Li Ba-fang
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
J Zhejiang Univ Sci B. 2009 Aug;10(8):572-9. doi: 10.1631/jzus.B0920081.
To optimize the hydrolysis conditions to prepare hydrolysates of jellyfish umbrella collagen with the highest hydroxyl radical scavenging activity, collagen extracted from jellyfish umbrella was hydrolyzed with trypsin, and response surface methodology (RSM) was applied. The optimum conditions obtained from experiments were pH 7.75, temperature (T) 48.77 degrees C, and enzyme-to-substrate ratio ([E]/[S]) 3.50%. The analysis of variance in RSM showed that pH and [E]/[S] were important factors that significantly affected the process (P<0.05 and P<0.01, respectively). The hydrolysates of jellyfish umbrella collagen were fractionated by high performance liquid chromatography (HPLC), and three fractions (HF-1>3000 Da, 1000 Da<HF-2<3000 Da, and HF-3<1000 Da) were collected. The HF-2 fraction had the highest hydroxyl radical scavenging activity with the highest yield compared with the other two fractions. Furthermore, HF-2 also showed the strongest Cu(2+)-chelating ability and the best tyrosinase-inhibitory activity.
为优化水解条件以制备具有最高羟自由基清除活性的海蜇伞胶原蛋白水解产物，用胰蛋白酶对从海蜇伞中提取的胶原蛋白进行水解，并应用响应面法（RSM）。实验得到的最佳条件为pH 7.75、温度（T）48.77℃和酶与底物比（[E]/[S]）3.50%。RSM的方差分析表明，pH和[E]/[S]是显著影响该过程的重要因素（分别为P<0.05和P<0.01）。海蜇伞胶原蛋白水解产物通过高效液相色谱（HPLC）进行分离，收集了三个级分（HF-1>3000 Da、1000 Da<HF-2<3000 Da和HF-3<1000 Da）。与其他两个级分相比，HF-2级分具有最高的羟自由基清除活性和最高的产率。此外，HF-2还表现出最强的Cu(2+)螯合能力和最佳的酪氨酸酶抑制活性。
