Karlinsey Robert L, Mackey Allen C, Stookey George K
Indiana Nanotech, LLC, 351 West 10th Street, Suite 309, Indianapolis, IN 46202, USA.
Am J Dent. 2009 Jun;22(3):185-8.
To determine the in vitro remineralization potential of a new calcium phosphate technology in a 1000 ppm F system.
3 mm diameter bovine enamel specimens were mounted, ground and polished, and softened in a carbopol-lactic acid solution (pH = 5.0) for 36 hours at 37 degrees C. Specimens were then measured for baseline Vickers microhardness and stratified (N = 10, mean VHN = 35) into the following groups: Group A: distilled water (negative control); Group B: MI Paste Plus (900 ppm F); Group C: Theramed SOS (1450 ppm F); Group D: "control" dentifrice (1000 ppm F); and, Group E: "test" dentifrice (1000 ppm F) admixed with a new functionalized calcium phosphate system. The groups were then cycled for 10 days in a pH cycling model consisting of four 2-minute treatment periods (diluted 1:3 with distilled water) and one 4-hour acid challenge (carbopol-lactic acid, pH = 5.0) per day. Between these events, specimens were immersed in artificial saliva (pH = 7.0). After 10 days of cycling, the specimens were measured for Vickers surface microhardness and were subsequently microdrilled, with the powder measured for fluoride content using a calibrated fluoride-sensitive electrode.
Significant differences resulted between the distilled water and fluoride-containing groups. Among the fluoride-containing groups, Group B demonstrated statistically low levels of enamel fluoride deposition and deltaVHN, while Group E statistically outperformed Group D. Among the groups with different calcium systems (Groups B, C, and E), Groups C and E were found to be statistically equivalent and superior to Group B with respect to both bioavailable fluoride and deltaVHN. Based on our results, these data demonstrated the combination of a new calcium phosphate technology plus 1000 ppm F, produced significantly greater remineralization relative to both the 1000 ppm F test dentifrice and MI Paste Plus, and was statistically equivalent to Theramed SOS.
确定一种新型磷酸钙技术在1000 ppm氟体系中的体外再矿化潜力。
将直径3 mm的牛牙釉质标本进行安装、研磨和抛光，然后在37℃下于卡波姆 - 乳酸溶液（pH = 5.0）中软化36小时。接着测量标本的基线维氏显微硬度，并将其分层（N = 10，平均维氏硬度值 = 35）分为以下几组：A组：蒸馏水（阴性对照）；B组：MI Paste Plus（900 ppm氟）；C组：Theramed SOS（1450 ppm氟）；D组：“对照”牙膏（1000 ppm氟）；E组：与新型功能化磷酸钙体系混合的“测试”牙膏（1000 ppm氟）。然后将这些组在一个pH循环模型中循环10天，该模型每天包括四个2分钟的处理期（用蒸馏水1:3稀释）和一个4小时的酸挑战（卡波姆 - 乳酸，pH = 5.0）。在这些过程之间，将标本浸泡在人工唾液（pH = 7.0）中。循环10天后，测量标本的维氏表面显微硬度，随后进行微钻孔，用校准的氟敏感电极测量粉末中的氟含量。
蒸馏水组与含氟组之间存在显著差异。在含氟组中，B组的牙釉质氟沉积和维氏硬度变化值在统计学上处于低水平，而E组在统计学上优于D组。在具有不同钙体系的组（B组、C组和E组）中，发现C组和E组在生物可利用氟和维氏硬度变化值方面在统计学上相当且优于B组。基于我们的结果，这些数据表明新型磷酸钙技术与1000 ppm氟的组合相对于1000 ppm氟测试牙膏和MI Paste Plus产生了显著更大的再矿化，并且在统计学上与Theramed SOS相当。
