Karlinsey Robert L, Mackey Allen C, Stookey George K, Pfarrer Aaron M
Indiana Nanotech, LLC, 351 West 10th Street, Suite 309, Indianapolis, IN 46202, USA.
Am J Dent. 2009 Jun;22(3):180-4.
To determine the fluoride dose response of experimental NaF dentifrices containing a prospective calcium phosphate technology, along with the corresponding relative enamel and dentin abrasion values.
3 mm diameter bovine enamel specimens were mounted, ground and polished, and softened in a carbopol-lactic acid solution (pH = 5.0) for 36 hours at 37 degrees C. Specimens were then measured for baseline Vickers microhardness and stratified (N = 18, mean VHN = 33) into eight groups. These groups consisted of a placebo paste, four test dentifrices (A, B, C, D) with three of the four (A, B, C) containing a promising calcium phosphate ingredient, Crest Cavity Protection, MI Paste Plus, and PreviDent Booster 5000. The groups were cycled in a lesion reversal pH cycling model consisting of four 2-minute treatment periods (diluted 1:3 with DI water) and one 4-hour acid challenge (carbopol-lactic acid, pH = 5.0) per day. Between these events, specimens were immersed in artificial saliva (pH = 7.0). After 20 days of cycling, the specimens were microdrilled and analyzed for fluoride content, and also measured for Vickers surface microhardness after 10 and 20 days of cycling and after a 2-hour and 16-hour post-cycle acid challenge (carbopol-lactic acid, pH = 5.0). Separately, relative dentin and enamel abrasion (RDA and REA) were performed using the ADA recommended radiotracer method.
A fluoride dose response was observed for the test dentifrices after 10 and 20 days of pH cycling, with test dentifrice C promoting the highest remineralization among the groups while both the MI Paste Plus and PreviDent systems provide the least remineralization (one-way ANOVA, SNK, P < 0.05). With respect to enamel fluoride uptake, the group facilitating the highest incorporation of fluoride into the enamel lesion was test dentifrice C, while the least effective NaF system was the MI Paste Plus (one-way ANOVA, SNK, P < 0.05). In terms of formulation abrasion, the REA scores were similar among the test dentifrices, MI Paste Plus, and PreviDent and compared favorably to the ADA reference material score (one-way ANOVA, SNK, P < 0.05); relative to the ADA reference material RDA score, the data indicate that MI Paste Plus was essentially non-abrasive, while PreviDent was significantly more abrasive to dentin (one-way ANOVA, SNK, P < 0.05). Altogether, these data show the developmental test dentifrices demonstrate a fluoride dose response and show great promise in remineralizing white-spot enamel lesions relative to MI Paste Plus and PreviDent.
确定含有前瞻性磷酸钙技术的实验性氟化钠牙膏的氟剂量反应，以及相应的相对牙釉质和牙本质磨损值。
将直径3毫米的牛牙釉质标本安装、研磨和抛光，在37摄氏度的卡波姆 - 乳酸溶液（pH = 5.0）中软化36小时。然后测量标本的基线维氏显微硬度，并将其分层（N = 18，平均维氏硬度值 = 33）为八组。这些组包括一种安慰剂牙膏、四种测试牙膏（A、B、C、D），其中四种中的三种（A、B、C）含有一种有前景的磷酸钙成分、佳洁士防蛀牙膏、MI糊剂加强版和Prevident 5000。这些组在一个病变逆转pH循环模型中循环，该模型包括每天四个2分钟的治疗期（用去离子水1:3稀释）和一个4小时的酸挑战（卡波姆 - 乳酸，pH = 5.0）。在这些过程之间，将标本浸入人工唾液（pH = 7.0）中。循环20天后，对标本进行微钻孔并分析氟含量，并且在循环10天和20天后以及循环后2小时和16小时的酸挑战（卡波姆 - 乳酸，pH = 5.0）后测量维氏表面显微硬度。另外，使用美国牙科协会推荐的放射性示踪剂方法进行相对牙本质和牙釉质磨损（RDA和REA）测试。
在pH循环10天和20天后，观察到测试牙膏的氟剂量反应，测试牙膏C在各组中促进了最高的再矿化，而MI糊剂加强版和Prevident系统提供的再矿化最少（单因素方差分析，SNK检验，P < 0.05）。关于牙釉质氟摄取，促进氟最高程度地掺入牙釉质病变的组是测试牙膏C，而效果最差的氟化钠系统是MI糊剂加强版（单因素方差分析，SNK检验，P < 0.05）。在配方磨损方面，测试牙膏、MI糊剂加强版和Prevident的REA评分相似，并且与美国牙科协会参考材料评分相比良好（单因素方差分析，SNK检验，P < 0.05）；相对于美国牙科协会参考材料的RDA评分，数据表明MI糊剂加强版基本无磨损，而Prevident对牙本质的磨损明显更大（单因素方差分析，SNK检验，P < 0.05）。总之，这些数据表明开发中的测试牙膏表现出氟剂量反应，并且相对于MI糊剂加强版和Prevident在使白斑牙釉质病变再矿化方面显示出很大的前景。
