Premnath Parvathy, John Joseph, Manchery Nithin, Subbiah Gireesh K, Nagappan Nagappan, Subramani Prabhu
Public Health Dentistry, Asan Memorial Dental College and Hospital, Chengalpattu, IND.
Public Health Dentistry, Saveetha Dental College, Saveetha University, Chennai, IND.
Cureus. 2019 Sep 17;11(9):e5686. doi: 10.7759/cureus.5686.
Background Remineralizing agents demonstrate potential to reverse early carious lesions. Theobromine containing dentifrices claim to remineralize enamel lesions effectively. The aim of this in-vitro study was to evaluate and compare the remineralization potential of dentifrices containing theobromine, 0.21% sodium fluoride (NaF) with functionalized tricalcium phosphate (f-TCP) and amine fluoride on artificial enamel caries. Materials and methods Sound extracted human premolars were demineralized to produce deep artificial carious lesions. The teeth were sectioned longitudinally and allocated to three treatment groups with nine specimens in each group: Group A (NaF + f-TCP), Group B (amine fluoride), and Group C (theobromine). The specimens were then subjected to pH cycling for seven days. Confocal laser scanning microscopy (CLSM) was utilized to record the patterns of demineralization and remineralization. One-way ANOVA and paired t-test were used to analyze changes in lesion depth. The level of significance was set at p<0.05. Results All three dentifrices effectively remineralized artificial carious lesions (paired t-test, p<0.001). Of the groups, Group A (54.97%) reported the highest percentage change in lesion depth values followed closely by Group B (51.51%) and Group C (31.71%), respectively. Conclusion Within this in-vitro study, theobromine containing dentifrice was effective in remineralizing lesions of enamel. However, theobromine demonstrated less remineralization potential in comparison to dentifrices containing NaF + f-(TCP) and amine fluoride.
背景 再矿化剂显示出逆转早期龋损的潜力。含可可碱的牙膏声称能有效使牙釉质龋损再矿化。本体外研究的目的是评估和比较含可可碱、0.21% 氟化钠（NaF）与功能化磷酸三钙（f-TCP）以及胺氟化物的牙膏对人工牙釉质龋的再矿化潜力。材料与方法 提取完好的人类前磨牙并进行脱矿以产生深部人工龋损。将牙齿纵向切片并分为三个治疗组，每组九个标本：A组（NaF + f-TCP）、B组（胺氟化物）和C组（可可碱）。然后将标本进行为期七天的pH循环。利用共聚焦激光扫描显微镜（CLSM）记录脱矿和再矿化模式。采用单因素方差分析和配对t检验分析病变深度的变化。显著性水平设定为p<0.05。结果 所有三种牙膏均能有效使人工龋损再矿化（配对t检验，p<0.001）。在各治疗组中，A组（54.97%）的病变深度值变化百分比最高，紧随其后的是B组（51.51%）和C组（31.71%）。结论 在本体外研究中，含可可碱的牙膏对牙釉质龋损具有再矿化作用。然而，与含NaF + f-TCP和胺氟化物的牙膏相比，可可碱的再矿化潜力较小。
