膳食补充剂Meltdown对男性和女性儿茶酚胺分泌、脂肪分解标志物及代谢率的影响：一项随机、安慰剂对照、交叉研究。
Effect of the dietary supplement Meltdown on catecholamine secretion, markers of lipolysis, and metabolic rate in men and women: a randomized, placebo controlled, cross-over study.
作者信息
Bloomer Richard J, Canale Robert E, Blankenship Megan M, Hammond Kelley G, Fisher-Wellman Kelsey H, Schilling Brian K
机构信息
Cardiorespiratory/Metabolic Laboratory, Department of Health and Sport Sciences, University of Memphis, Memphis, TN, USA.
出版信息
Lipids Health Dis. 2009 Aug 5;8:32. doi: 10.1186/1476-511X-8-32.
BACKGROUND
We have recently reported that the dietary supplement Meltdown increases plasma norepinephrine (NE), epinephrine (EPI), glycerol, free fatty acids (FFA), and metabolic rate in men. However, in that investigation measurements ceased at 90 minutes post ingestion, with values for blood borne variables peaking at this time. It was the purpose of the present investigation to extend the time course of measurement to 6 hours, and to include women within the design to determine if sex differences to treatment exist.
METHODS
Ten men (24 +/- 4 yrs) and 10 women (22 +/- 2 yrs) ingested Meltdown or a placebo, using a randomized, cross-over design with one week separating conditions. Blood samples were collected immediately before supplementation and at one hour intervals through 6 hours post ingestion. A standard meal was provided after the hour 3 collection. Samples were assayed for EPI, NE, glycerol, and FFA. Five minute breath samples were collected at each time for measurement of metabolic rate and substrate utilization. Area under the curve (AUC) was calculated. Heart rate and blood pressure were recorded at all times. Data were also analyzed using a 2 (sex) x 2 (condition) x 7 (time) repeated measures analysis of variance, with Tukey post hoc testing.
RESULTS
No sex x condition interactions were noted for AUC for any variable (p > 0.05). Hence, AUC data are collapsed across men and women. AUC was greater for Meltdown compared to placebo for EPI (367 +/- 58 pg x mL(-1) x 6 hr(-1) vs. 183 +/- 27 pg x mL(-1) x 6 hr(-1); p = 0.01), NE (2345 +/- 205 pg x mL(-1) x 6 hr(-1) vs. 1659 +/- 184 pg x mL(-1) x 6 hr(-1); p = 0.02), glycerol (79 +/- 8 microg x mL)-1) x 6 hr(-1) vs. 59 +/- 6 microg x mL(-1) x 6 hr(-1); p = 0.03), FFA (2.46 +/- 0.64 mmol x L(-1) x 6 hr(-1) vs. 1.57 +/- 0.42 mmol x L(-1) x 6 hr(-1); p = 0.05), and kilocalorie expenditure (439 +/- 26 kcal x 6 hrs(-1) vs. 380 +/- 14 kcal x 6 hrs(-1); p = 0.02). No effect was noted for substrate utilization (p = 0.39). Both systolic and diastolic blood pressure (p < 0.0001; 1-16 mmHg), as well as heart rate (p = 0.01; 1-9 bpm) were higher for Meltdown. No sex x condition x time interactions were noted for any variable (p > 0.05).
CONCLUSION
Ingestion of Meltdown results in an increase in catecholamine secretion, lipolysis, and metabolic rate in young men and women, with a similar response for both sexes. Meltdown may prove to be an effective intervention strategy for fat loss, assuming individuals are normotensive and their treatment is monitored by a qualified health care professional.
背景
我们最近报告称，膳食补充剂“极速燃脂”（Meltdown）可提高男性的血浆去甲肾上腺素（NE）、肾上腺素（EPI）、甘油、游离脂肪酸（FFA）水平以及代谢率。然而，在该研究中，测量在摄入后90分钟停止，此时血源性变量的值达到峰值。本研究的目的是将测量时间延长至6小时，并在研究设计中纳入女性，以确定对该治疗是否存在性别差异。
方法
10名男性（24±4岁）和10名女性（22±2岁）采用随机交叉设计，在两种情况之间间隔一周，分别摄入“极速燃脂”或安慰剂。在补充前及摄入后每小时采集一次血样，直至6小时。在3小时采集后提供标准餐。对血样检测EPI、NE、甘油和FFA。每次采集5分钟的呼吸样本，用于测量代谢率和底物利用情况。计算曲线下面积（AUC）。随时记录心率和血压。数据还采用2（性别）×2（情况）×7（时间）重复测量方差分析，并进行Tukey事后检验。
结果
任何变量的AUC均未发现性别×情况交互作用（p>0.05）。因此，将男性和女性的AUC数据合并。与安慰剂相比，“极速燃脂”组的EPI（367±58 pg·mL⁻¹·6 hr⁻¹ 对 183±27 pg·mL⁻¹·6 hr⁻¹；p = 0.01）、NE（2345±205 pg·mL⁻¹·6 hr⁻¹ 对 1659±184 pg·mL⁻¹·6 hr⁻¹；p = 0.02）、甘油（79±8 μg·mL⁻¹·6 hr⁻¹ 对 59±6 μg·mL⁻¹·6 hr⁻¹；p = 0.03）、FFA（2.46±0.64 mmol·L⁻¹·6 hr⁻¹ 对 1.57±0.42 mmol·L⁻¹·6 hr⁻¹；p = 0.05）和千卡消耗量（439±26 kcal·6 hr⁻¹ 对 380±14 kcal·6 hr⁻¹；p = 0.02）的AUC更大。未发现对底物利用有影响（p = 0.39）。“极速燃脂”组的收缩压和舒张压（p<0.0001；升高1 - 16 mmHg）以及心率（p = 0.0
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